missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Immunoassay Reagents and Kits
  4. ELISA Substrates and Reagents
  5. Thermo Scientific™ PNPP Substrates

Thermo Scientific™ PNPP Substrates

Catalog No. PI37621
Change view
Click to view available options
Format:
Kit
Liqiuid
Powder
Tablet
4 product options available for selection
Product selection table with 4 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Format
PI37621 Liqiuid
PI34045 Powder
PI34047 Tablet
PI37620 Kit
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 4 options available.
4 options
Catalog No. PI37621 Supplier Thermo Scientific™ Supplier No. 37621
Only null left

Description

Thermo Scientific™ Pierce PNPP Substrates are powders, tablets, solutions and complete kits of p-nitrophenyl phosphate, a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.

PNPP (p-Nitrophenyl Phosphate, Disodium Sal) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm. Pierce PNPP is available in four formats: dry crystalline powder, single-use tablets, a stable substrate solution, and a complete kit containing substrate solution and diethanolamine buffer.

Thermo Scientific Pierce PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed. This reaction product absorbs light at 405 nm.

Features of PNPP (p-nitrophenyl phosphate):

  • One-component alkaline phosphatase (AP) substrate
  • Absorbance maximum of 405 nm
  • Develops a yellow hue in solution upon reaction with AP
  • Safer to use—contains a non-mercury, non-azide preservative

The substrate is offered in several formats:

PNPP Substrate

  • Powder Form

PNPP Substrate Tablets

  • 5 mg tablets with an inert filler as the balance

PNPP Substrate Kit

  • PNPP tablets and a Diethanolamine Buffer to yield more than one liter of substrate. The Diethanolamine Substrate Buffer (Cat. No. 34064) is also provided individually as a 5X concentrate.

1-Step™ PNPP Substrate Solution

  • Circumvents this time-consuming and variable introducing step by providing a single component PNPP substrate. This substrate is stable for 12 months at 2–8°C and can be stopped with conventional methods.

Specifications

Description 1-Step™ PNPP Substrate Solution
Product Type ELISA Substrate
Quantity 100 mL
Detection Method Colorimetric
Form Liquid
Format Liqiuid
Product Line 1-Step
Substrate Properties Chemical Substrate
Substrate Type Alkaline Phosphatase
Content And Storage Upon receipt store product at 4°C.
Shipping Condition Ambient
Show More Show Less

Frequently Asked Questions (FAQs)

What alkaline phosphatase substrates do you offer?

For colorimetric alkaline phosphatase substrates, we offer the following products:

Novex AP Chromogenic Substrate (BCIP/ NBT) (Cat. No. WP20001)

1-Step NBT/BCIP Substrate Solution (Cat. No. 34042)

1-Step PNPP Substrates Solution (Cat. No. 37621)

NBT and BCIP are also available as standalone products:

NBT Substrate Powder (nitro-blue tetrazolium chloride) (Cat. No. 34035)

BCIP Substrate Powder (5-bromo-4-chloro-3'-indolylphosphate p-toluidine) (Cat. No. 34040)

For chemiluminescent alkaline substrates, we offer the following products:

Novex AP Chemiluminescent Substrate (CDP-Star) (Cat. No. WP20002)

NovaBright Phospha-Light EXP Assay Kit for SEAP (Secreted Placental Alkaline Phosphatase) Reporter Gene Detection (Cat. Nos. N10577, N10578)