Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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Invitrogen™ Human MCP-2 ProQuantum Immunoassay Kit
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ELISA
| Immunoassay Kit Format | Proximity-based Immunoassay |
|---|---|
| Target Species | Human |
| Conjugate | FAM™ |
| Incubation Time | 1 hr. |
| Total Assay Time | 2 hrs |
| Sample Volume | Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL |
| Assay Sensitivity | <0.01 pg/mL |
| Storage Requirements | Frozen at -20°C |
| Assay Range | 0.0128 to 5000 pg/mL |
| For Use With (Application) | Protein Quantitation |
| Test Time | 30 mins |
| Detection Method | Taqman™ Real-time PCR |
| Accession Number | P80075 |
| Regulatory Status | RUO |
| Target | CCL8 (MCP2) |
| Kit Contents | Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase |
| For Use With (Equipment) | qPCR Instruments |
| Product Type | ProQuantum |
| Gene ID (Entrez) | 6355 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | FAM™ |
| Protein Family | Cytokines & Receptors |
|---|---|
| Conjugate | HRP |
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | IFN-G,IFN-gamma |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 100 μL |
| Storage Requirements | 2°C to 8°C |
| Research Discipline | Immunology |
| Assay Range | 0.102 to 25 ng/mL |
| For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| Test Time | 1 hrs 20 mins |
| Assay | Solid-phase sandwich ELISA |
| Accession Number | P42161 |
| Regulatory Status | RUO |
| Target | IFN gamma |
| Specificity | This ELISA antibody pair detects canine IFN-gamma, no cross-reactivity was observed with recombinant human, mouse, rat, cotton rat, feline, porcine, equine or bovine IFN-gamma. |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 403801 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | IFN-GIFN-gamma |
| Shipping Condition | Wet Ice |
|---|---|
| Content And Storage | 2°C to 8°C |
| Host Species | Human |
| Conjugate | HRP |
| Interassay CV | <10% |
| Intraassay CV | <10 |
| Total Assay Time | 1 hrs 30 mins |
| Sample Volume | Plasma 5 μL, Serum 5 μL |
| Protein | SARS-CoV-2 Spike Protein RBD |
| Test Time | 40 mins |
| Regulatory Status | RUO |
| Target | SARS-CoV-2 Spike Protein RBD |
| Kit Contents | Pre-coated 96 well plate,Control,SA-HRP Conjugate,Assay Buffer concentrate,Wash Buffer concentrate,Substrate solution,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Sample Type | Plasma,Serum |
Invitrogen™ IgG3 Mouse ELISA Standard
This product is a component of a respective eBioscience Kit. Please refer to the relevant manual for each product on our website.
Invitrogen™ IgA Rat ELISA Standard (for Uncoated ELISA Kit)
This product is a component of a respective eBioscience Kit. Please refer to the relevant manual for each product on our website.
Invitrogen™ IgG1 Human ELISA Standard (for Uncoated ELISA Kit)
This product is a component of a respective eBioscience Kit. Please refer to the relevant manual for each product on our website.
Invitrogen™ IgG2b Rat ELISA Standard (for Uncoated ELISA Kit)
This product is a component of a respective eBioscience Kit. Please refer to the relevant manual for each product on our website.
| Target Species | Rat |
|---|---|
| Conjugate | HRP |
| Total Assay Time | 24 hrs 30 mins |
| Sample Volume | Plasma 10 μL, Serum 10 μL, Supernatant 10 μL |
| Assay Sensitivity | 0.39 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.39 to 25 ng/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 45 mins |
| Regulatory Status | RUO |
| Target | Rat IgG2a |
| Kit Contents | Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Rat IgG2a protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Sample Type | Plasma,Serum,Supernatant |
| Synonym | Immunoglobulin G2a |
| For Use With (Equipment) | Colorimetric Microplate Reader |
|---|---|
| Gene ID (Entrez) | 26419, 26420, 5599, 5601 |
| For Use With (Application) | ELISA |
| Target Species | Mouse |
|---|---|
| Conjugate | HRP |
| Total Assay Time | 24 hrs 30 mins |
| Sample Volume | Plasma 10 μL, Serum 10 μL, Supernatant 50 μL |
| Assay Sensitivity | 4 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 4 to 250 ng/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 45 mins |
| Regulatory Status | RUO |
| Target | Mouse IgE |
| Kit Contents | Capture Antibody: Pre-titrated,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86),Purified antibody,Biotin-conjugated antibody,Biotin-conjugated antibody,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 380792 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | Immunoglobulin E |
| Target Species | Rat |
|---|---|
| Conjugate | HRP |
| Total Assay Time | 24 hrs 30 mins |
| Sample Volume | Plasma 10 μL, Serum 10 μL, Supernatant 50 μL |
| Assay Sensitivity | 1.6 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 1.6 to 100 ng/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 45 mins |
| Regulatory Status | RUO |
| Target | Rat IgG |
| Kit Contents | Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | Immunoglobulin G |
| Target Species | Rat |
|---|---|
| Conjugate | HRP |
| Total Assay Time | 24 hrs 30 mins |
| Sample Volume | Plasma 10 μL, Serum 10 μL, Supernatant 10 μL |
| Assay Sensitivity | 0.39 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.39 to 25 ng/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 45 mins |
| Regulatory Status | RUO |
| Target | Rat IgG2a |
| Kit Contents | Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Sample Type | Plasma,Serum,Supernatant |
| Synonym | Immunoglobulin G2a |
| Target Species | Rat |
|---|---|
| Conjugate | HRP |
| Total Assay Time | 24 hrs 30 mins |
| Sample Volume | Plasma 10 μL, Serum 10 μL, Supernatant 10 μL |
| Assay Sensitivity | 0.3125 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.3125 to 20 ng/mL |
| For Use With (Application) | ELISA |
| Test Time | 1 hrs 45 mins |
| Regulatory Status | RUO |
| Target | Rat IgM |
| Kit Contents | Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 299357 |
| Sample Type | Plasma,Serum,Supernatant |
| Synonym | Immunoglobulin M |