Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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Invitrogen™ Human MCP-2 ProQuantum Immunoassay Kit
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ELISA
| Immunoassay Kit Format | Proximity-based Immunoassay |
|---|---|
| Target Species | Human |
| Conjugate | FAM™ |
| Incubation Time | 1 hr. |
| Total Assay Time | 2 hrs |
| Sample Volume | Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL |
| Assay Sensitivity | <0.01 pg/mL |
| Storage Requirements | Frozen at -20°C |
| Assay Range | 0.0128 to 5000 pg/mL |
| For Use With (Application) | Protein Quantitation |
| Test Time | 30 mins |
| Detection Method | Taqman™ Real-time PCR |
| Accession Number | P80075 |
| Regulatory Status | RUO |
| Target | CCL8 (MCP2) |
| Kit Contents | Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase |
| For Use With (Equipment) | qPCR Instruments |
| Product Type | ProQuantum |
| Gene ID (Entrez) | 6355 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | FAM™ |
| Protein Family | Cytokines & Receptors |
|---|---|
| Conjugate | HRP |
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | IFN-G,IFN-gamma |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 100 μL |
| Storage Requirements | 2°C to 8°C |
| Research Discipline | Immunology |
| Assay Range | 0.102 to 25 ng/mL |
| For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| Test Time | 1 hrs 20 mins |
| Assay | Solid-phase sandwich ELISA |
| Accession Number | P42161 |
| Regulatory Status | RUO |
| Target | IFN gamma |
| Specificity | This ELISA antibody pair detects canine IFN-gamma, no cross-reactivity was observed with recombinant human, mouse, rat, cotton rat, feline, porcine, equine or bovine IFN-gamma. |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 403801 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | IFN-GIFN-gamma |
| Shipping Condition | Wet Ice |
|---|---|
| Content And Storage | 2°C to 8°C |
| Host Species | Human |
| Conjugate | HRP |
| Interassay CV | <10% |
| Intraassay CV | <10 |
| Total Assay Time | 1 hrs 30 mins |
| Sample Volume | Plasma 5 μL, Serum 5 μL |
| Protein | SARS-CoV-2 Spike Protein RBD |
| Test Time | 40 mins |
| Regulatory Status | RUO |
| Target | SARS-CoV-2 Spike Protein RBD |
| Kit Contents | Pre-coated 96 well plate,Control,SA-HRP Conjugate,Assay Buffer concentrate,Wash Buffer concentrate,Substrate solution,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Sample Type | Plasma,Serum |
| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | Il4 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 8 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 8 to 2,000 pg/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | B cell growth factor 1; B |
| Regulatory Status | RUO |
| Target | IL-4 |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 280824 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | IL21 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.41 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.41 to 100 ng/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | CVID11; C x 2; il 21; Il21; IL-21; ILN; Interleukin; interleukin 21; interleukin 21-like; Interleukin21; interleukin-21; interleukin-21 isoform; LOC100344172; porcine interleukin-21; Za11 |
| Regulatory Status | RUO |
| Target | IL-21 |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 378475 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | INS |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 4.5 μIU/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 4.5 to 300 μIU/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | AA986540; IDDM; IDDM1; IDDM2; ILPR; INS; Ins1; Ins-1; Ins2; Ins-2; Ins2-rs1; InsII; Insulin; insulin 1; insulin 2; Insulin A chain; Insulin B chain; insulin I; insulin II; insulin-1; Insulin-1 A chain; Insulin-1 B chain; Insulin-2; Insulin-2 A chain; Insulin-2 B chain; IRDN; Mody; MODY10; Mody4; preproinsulin; proinsulin |
| Regulatory Status | RUO |
| Target | Insulin |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 280829 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | FGF1 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 0.2 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.205 to 50 ng/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | acidic eye-derived fibroblast growth factor; acidic eye-derived growth factor II; Acidic fibroblast growth factor; aFGF; beta-endothelial cell growth factor; Dffrx; ECGF; ECGFA; ECGFB; ECGF-beta; EDGF; EDGF II; Endothelial cell growth factor; Endothelial cell growth factor alpha; Endothelial cell growth factor beta; endothelial cell growth factor beta and alpha chains; endothelial cell growth factor, alpha; endothelial cell growth factor, beta; endothelial growth factor; Fam; FGF; FGF1; Fgf-1; FGF1 protein; Fgfa; FGF-alpha; Fibroblast growth factor; fibroblast growth factor 1; fibroblast growth factor 1 (acidic); Fibroblast growth factor 1 (heparin binding); fibroblast growth factor, acidic; GLIO703; HBGF1; HBGF-1; heparin-binding growth factor; heparin-binding growth factor 1; H-FGF-a; M-FGF-a; Prostatropin; unnamed protein product |
| Regulatory Status | RUO |
| Target | FGF1 |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 281160 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | IGF1 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 1.23 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 1.23 to 300 ng/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | C730016P09Rik; class 1 insulin-like growth factor I preproprotein; class 2 insulin-like growth factor I preproprotein; H-IGF-1; IBP1; IGF; IGF1; Igf-1; IGF1A; IGFI; Igf-I; IGFIa; IGF-IA; IGF-IB; insulin growth factor-1; Insulin like growth factor; insulin like growth factor 1; insulin-like growth factor 1; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor I; insulin-like growth factor I (somatomedin C); insulin-like growth factor IB; mechano growth factor; MGF; M-IGF-1; OTTHUMP00000195084; prepro-IGF-I; prepro-insulin-like growth factor I; R-IGF-1; somatomedin; Somatomedin C; Somatomedin-C |
| Regulatory Status | RUO |
| Target | IGF1 |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 281239 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Protein Family | Other Proteins |
|---|---|
| Conjugate | HRP |
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | IL2 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 100 μL |
| Assay Sensitivity | 0.2 ng/mL |
| Storage Requirements | 2°C to 8°C |
| Research Discipline | Immunology |
| Assay Range | 0.273 to 200 ng/mL |
| For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| Test Time | 1 hrs 20 mins |
| Assay | Solid-phase sandwich ELISA |
| Accession Number | P05016 |
| Regulatory Status | RUO |
| Target | IL-2 |
| Specificity | Detects bovine IL-2 in. this Sandwich ELISA. It shows less than 0.2% cross-reactivity with the recombinant human IL-2, recombinant mouse IL-2, recombinant rat IL-2, recombinant cotton rat IL-2, recombinant canine IL-2, recombinant equine IL-2, recombinant feline IL-2. |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 280822 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | BOS |
| Protein Family | Kinase & Inhibitors |
|---|---|
| Conjugate | HRP |
| Intraassay CV | <10 |
| Detectable Analytes | TNF alpha |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 100 μL |
| Assay Sensitivity | 0.1 ng/mL |
| Research Discipline | Extracellular Matrix & Cell Adhesion |
| For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| Test Time | 1 hrs 20 mins |
| Detection Method | Colorimetric |
| Assay | Solid-phase sandwich ELISA |
| Target | TNF alpha |
| Specificity | This ELISA antibody pair detects bovine TNF-alpha. Other species not determined |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Label or Dye | HRP |
| Calibration Range | 0.1 ng/ml to 30 ng/ml |
| Interassay CV | <12% |
| Incubation Time | 5 hr. |
| Gene Symbol | TNF-a,TNF-alpha,TNFa |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 0.123 to 30 ng/mL |
| Accession Number | Q06599 |
| Regulatory Status | RUO |
| Product Type | ELISA |
| Gene ID (Entrez) | 280943 |
| Sample Type | Plasma,Serum,Supernatant |
| Synonym | BOS |
| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | Ccl2 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 9 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 9 to 700 pg/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | Acidic seminal fluid protein; AI323594; C-C motif chemokine 2; C-C motif chemokine ligand 2; Ccl2; chemokine (C-C motif) ligand 2; GDCF-2; HC11; H-MCP-1; HSMCR30; immediate-early serum-responsive JE protein; immediate-early serum-responsive protein JE; Je; MCAF; MCP1; MCP-1; MCP1A; MCP-1 A; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; monocyte chemotactic protein 1 A; Monocyte secretory protein JE; monocyte-chemoattractant protein-1 precursor; platelet-derived growth factor-inducible protein JE; RP23-350G1.3; Scya2; Sigje; small inducible cytokine A2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible gene JE; small-inducible cytokine A2; SMC-CF |
| Regulatory Status | RUO |
| Target | CCL2 (MCP-1) |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 403981 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | TIMP2 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 20 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 20 to 5,000 pg/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | collagenase inhibitor; CSC-21 K; D11Bwg1104e; DDC8; Metalloproteinase inhibitor 2; metalloproteinase inhibitor precursor; metalloproteinase inhibitor TIMP-2; similar to metalloproteinase inhibitor; testicular secretory protein Li 57; TIMP 2; TIMP metallopeptidase inhibitor 2; Timp2; TIMP-2; TIMP-2 protein; tissue inhibitor of matrix metalloproteinase-2; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinase-2; tissue inhibitor of metalloproteinases 2; tissue inhibitor of mettaloproteinase 2 |
| Regulatory Status | RUO |
| Target | TIMP2 |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 403633 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Conjugate | HRP |
|---|---|
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 50 μL |
| Assay Sensitivity | 10 pg/mL |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 10 to 2,500 pg/mL |
| Test Time | 1 hrs 20 mins |
| Accession Number | AD8; ARMD11; bA218C14.4 (cystatin C); cst3; CYSC; cystatin; cystatin 3; cystatin C; cystatin C (amyloid angiopathy and cerebral hemorrhage); Cystatin C (cysteine proteinase inhibitor); Cystatin3; cystatin-3; CystatinC; Cystatin-C; epididymis secretory protein Li 2; fb51d07; Gamma-trace; HEL-S-2; MGC117328; Neuroendocrine basic polypeptide; OTTHUMP00000030440; OTTHUMP00000164181; OTTHUMP00000164182; OTTMUSP00000016736; Post-gamma-globulin; RP23-250M11.9; wu:fb24g06; wu:fb51d07; wu:fc55f03; zgc:136227 |
| Regulatory Status | RUO |
| Target | Cystatin C |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |