Immunoassay Reagents and Kits
Kits, substrates, and reagents for a variety of immunoassay procedures, including ELISA, singleplex immunoassays, and multiplex immunoassays, that are ideal for the quantification of target antigens; substrates available in colorimetric, chemiluminescent, or chemifluorescent varieties.
We offer a variety of products for enzyme-linked immunosorbent assays (ELISA or EIA) including plates, reagents, and kits to help you detect and quantify peptides, proteins, antibodies, and hormones.
ELISA is an analytical method based on the highly specific interaction between an antigen and an antibody. For this assay, an antigen is attached to a solid surface, to which an antibody-enzyme complex is linked. When a substrate is added and incubated with the other materials, it produces a measurable product.
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Invitrogen™ Human MCP-2 ProQuantum Immunoassay Kit
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ELISA
| Immunoassay Kit Format | Proximity-based Immunoassay |
|---|---|
| Target Species | Human |
| Conjugate | FAM™ |
| Incubation Time | 1 hr. |
| Total Assay Time | 2 hrs |
| Sample Volume | Plasma 2-5 μL, Serum 2-5 μL, Supernatant 2-5 μL |
| Assay Sensitivity | <0.01 pg/mL |
| Storage Requirements | Frozen at -20°C |
| Assay Range | 0.0128 to 5000 pg/mL |
| For Use With (Application) | Protein Quantitation |
| Test Time | 30 mins |
| Detection Method | Taqman™ Real-time PCR |
| Accession Number | P80075 |
| Regulatory Status | RUO |
| Target | CCL8 (MCP2) |
| Kit Contents | Assay Dilution Buffer,Antibody-Conjugate A,Antibody-Conjugate B,Antibody-Conjugate Dilution Buffer,Standard,Master Mix,Ligase |
| For Use With (Equipment) | qPCR Instruments |
| Product Type | ProQuantum |
| Gene ID (Entrez) | 6355 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | FAM™ |
| Protein Family | Cytokines & Receptors |
|---|---|
| Conjugate | HRP |
| Interassay CV | <12% |
| Intraassay CV | <10 |
| Gene Symbol | IFN-G,IFN-gamma |
| Total Assay Time | 4 hrs 45 mins |
| Sample Volume | Plasma 50 μL, Serum 50 μL, Supernatant 100 μL |
| Storage Requirements | 2°C to 8°C |
| Research Discipline | Immunology |
| Assay Range | 0.102 to 25 ng/mL |
| For Use With (Application) | ELISA, Protein Assays, Protein Biology, Cancer Biology, Immunology, Neuroscience, Neurobiology, Protein Detection, Protein Analysis |
| Test Time | 1 hrs 20 mins |
| Assay | Solid-phase sandwich ELISA |
| Accession Number | P42161 |
| Regulatory Status | RUO |
| Target | IFN gamma |
| Specificity | This ELISA antibody pair detects canine IFN-gamma, no cross-reactivity was observed with recombinant human, mouse, rat, cotton rat, feline, porcine, equine or bovine IFN-gamma. |
| Kit Contents | Pre-coated 96 well plate,Standard,Assay Diluent concentrate,Biotinylated Detection Antibody,SAV-HRP,Wash Buffer,Chromogen,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 403801 |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| Synonym | IFN-GIFN-gamma |
| Shipping Condition | Wet Ice |
|---|---|
| Content And Storage | 2°C to 8°C |
| Host Species | Human |
| Conjugate | HRP |
| Interassay CV | <10% |
| Intraassay CV | <10 |
| Total Assay Time | 1 hrs 30 mins |
| Sample Volume | Plasma 5 μL, Serum 5 μL |
| Protein | SARS-CoV-2 Spike Protein RBD |
| Test Time | 40 mins |
| Regulatory Status | RUO |
| Target | SARS-CoV-2 Spike Protein RBD |
| Kit Contents | Pre-coated 96 well plate,Control,SA-HRP Conjugate,Assay Buffer concentrate,Wash Buffer concentrate,Substrate solution,Stop Solution,Adhesive Plate Covers |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Sample Type | Plasma,Serum |
Thermo Scientific™ CaptureSelect™ AAV8 Ligand Leakage ELISA
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Thermo Scientific™ CaptureSelect™ AAV8 Ligand Leakage ELISA is designed to detect possible leached ligand of adeno-associated viruses (AAV) purified using POROS CaptureSelect AAV8 Affinity Resin.
| For Use With (Equipment) | Microplate Reader |
|---|---|
| Product Line | CaptureSelect™ |
| Detection Method | Colorimetric |
Thermo Scientific™ CaptureSelect™ hCG Ligand Leakage ELISA
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Thermo Scientific™ CaptureSelect™ hCG Ligand Leakage ELISA is designed to detect possible leached ligand of human chorionic gonadotropin (hCG) purified using CaptureSelect hCG Affinity Matrix.
| For Use With (Equipment) | Microplate Reader |
|---|---|
| Product Line | CaptureSelect™ |
| Detection Method | Colorimetric |
Thermo Scientific™ CaptureSelect™ AAVX Ligand Leakage ELISA
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Thermo Scientific™ CaptureSelect™ AAVX Ligand Leakage ELISA is designed to detect possible leached ligand of adeno-associated viruses (AAV) purified using POROS CaptureSelect AAVX Affinity Matrix.
| Shipping Condition | Dry Ice |
|---|---|
| Product Line | CaptureSelect™ |
| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Gene Symbol | ERK,ERK-2,ERK2,ERT1,MAPK2,P42MAPK,PRKM1,PRKM2,p38,p40,p41,p41mapk,p42-MAPK,ERK-1,ERK1,ERT2,HS44KDAP,HUMKER1A,P44ERK1,P44MAPK,PRKM3,p44-ERK1,p44-MAPK |
| Total Assay Time | 1 hrs 10 mins |
| Sample Volume | Cell Lysate 20 μL |
| Storage Requirements | 2°C to 8°C |
| Test Time | 1 hrs 20 mins |
| Accession Number | P27361,P28482 |
| Regulatory Status | RUO |
| Target | ERK1/2 |
| Kit Contents | Capture Antibody,Detection Antibody Reagent,InstantOne™ Assay Plate,Cell Lysis Buffer,Enhancer Solution,Wash Buffer (10X),Substrate Reagent (TMB),Stop Solution,Positive Control Cell Lysate (lyophilized),Plate seal |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 5594, 5595 |
| Sample Type | Cell Lysates |
| Label or Dye | HRP |
| Target Species | Human |
|---|---|
| Conjugate | HRP |
| Gene Symbol | AKT,CWS6,PKB,PKB-ALPHA,PRKBA,RAC,RAC-ALPHA,HIHGHH,PKBB,PKBBETA,PRKBB,RAC-BETA,MPPH,MPPH2,PKB-GAMMA,PKBG,PRKBG,RAC-Pk.-gamma,RAC-gamma,STK-2 |
| Total Assay Time | 1 hrs 10 mins |
| Sample Volume | Cell Lysate 20 μL |
| Storage Requirements | 2°C to 8°C |
| Test Time | 1 hrs 20 mins |
| Accession Number | P31749 |
| Regulatory Status | RUO |
| Target | AKT1 |
| Kit Contents | Capture Antibody,Detection Antibody Reagent,InstantOne™ Assay Plate,Cell Lysis Buffer,Enhancer Solution,Wash Buffer (10X),Substrate Reagent (TMB),Stop Solution,Positive Control Cell Lysate (lyophilized),Plate seal |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Gene ID (Entrez) | 207 |
| Sample Type | Cell Lysates |
| Label or Dye | HRP |
| For Use With (Equipment) | Colorimetric Microplate Reader |
|---|---|
| Gene ID (Entrez) | 6776, 6777 |
| For Use With (Equipment) | Colorimetric Microplate Reader |
|---|---|
| Gene ID (Entrez) | 4086 |
| For Use With (Equipment) | Colorimetric Microplate Reader |
|---|---|
| Gene ID (Entrez) | 80381 |
| Assay Sensitivity | 0.16 ng/ml |
| Assay Range | 0.16 to 10 ng/mL |
| Target Species | Rat |
|---|---|
| Conjugate | HRP |
| Total Assay Time | 24 hrs 30 mins |
| Sample Volume | Plasma 10 μL, Serum 10 μL, Supernatant 50 μL |
| Assay Sensitivity | 16 ng/ml |
| Storage Requirements | 2°C to 8°C |
| Assay Range | 16 to 1,000 ng/mL |
| Test Time | 1 hrs 45 mins |
| Regulatory Status | RUO |
| Target | Rat IgA |
| Kit Contents | Capture Antibody: Pre-titrated,Purified antibody,Detection Antibody: Pre-titrated,HRP-conjugated antibody,Standard: Recombinant protein for generating standard curve and calibrating samples,10X Coating Buffer: Buffer for plating the Capture Antibody,5X ELISA/ELISPOT Diluent: Buffer for blocking and diluting the Detection Antibody and Enzyme,Substrate: 1X TMB Solution,Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards,96 Well Plate: High affinity 96-well plate (included with product Cat. Nos. ending in suffixes -22, -76, -86) |
| For Use With (Equipment) | Colorimetric Microplate Reader |
| Product Type | ELISA |
| Sample Type | Plasma,Serum,Supernatant |
| Label or Dye | HRP |
| For Use With (Equipment) | Colorimetric Microplate Reader |
|---|---|
| Assay Sensitivity | 1.6 ng/ml |
| Assay Range | 1.6 to 100 ng/mL |
| For Use With (Equipment) | Colorimetric Microplate Reader |
|---|---|
| Assay Sensitivity | 0.39 ng/ml |
| Assay Range | 0.39 to 25 ng/mL |
| For Use With (Equipment) | Colorimetric Microplate Reader |
|---|---|
| Assay Sensitivity | 0.39 ng/ml |
| Assay Range | 0.39 to 25 ng/mL |