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ELISA Substrates and Reagents

Colorimetric, chemiluminescent and chemifluorescent substrates for alkaline phosphatase (AP), horseradish peroxidase (HRP) and other enzyme reporter systems; includes several sensitive formulations of TMB for kinetic, competition or sandwich ELISA.
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1 – 15 of 69 results
1

Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions

Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.

Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Detection Method Colorimetric
2

Thermo Scientific™ OPD Substrate Tablets (o-phenylenediamine dihydrochloride)

Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.

3

Invitrogen™ eBioscience™ TMB Solution (1X)

Recommended for ELISA Ready-SET-Go!™ kits. eBioscience™ TMB ELISA Substrate Solution, 1x is a sensitive chromogenic substrate supplied as single ready-to-use solution.

4

Invitrogen™ CDP-Star™ Substrate (0.4 mM Ready-to-Use) with Enhancer  DFS Item

Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.

Shipping Condition Room Temperature
Content And Storage Store the product at 4°C.
Format Bottle
Form Liquid
Product Type ELISA Substrate with Enhancer
Substrate Type Alkaline Phosphatase
Quantity 100 mL
Product Line CDP-Star, NovaBright
Detection Method Chemiluminescence
5

Thermo Scientific™ ELISA Blocker Blocking Buffer

Increases signal-to-noise ratio and minimizes background

6

Thermo Scientific™ QuantaRed™ Enhanced Chemifluorescent HRP Substrate Kit

Enable sensitivity comparable to enhanced chemiluminescence (ECL) using the most sensitive fluorescent ELISA substrate for peroxidase (HRP).

7

Thermo Scientific™ OPD (o-phenylenediamine dihydrochloride)

Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.

8

Invitrogen™ Super AquaBlue ELISA Substrate

Enhanced ABTS substrate solution for ELISA which provides high signal and sensitivity, low background. Safe, stable and convenient to use. Oxidized by HRP-labeled probes, resulting in soluble, blue-green colored end product, whose absorption can be measured by spectrophotometers at 405nm.

Substrate Properties Chemical Substrate
Product Type ELISA Substrate
Substrate Type HRP (Horseradish Peroxidase)
Detection Method Colorimetric
9

Invitrogen™ CSPD™ Substrate (0.25 mM Ready-To-Use)  DFS Item

This CSPD™ substrate is a chemiluminescent alkaline phosphatase substrate, ready-to-use for protein or nucleic acid blotting on nitrocellulose membranes.

Shipping Condition Room Temperature
Content And Storage Store the product at 4°C.
Format Bottle
Form Liquid
Product Type ELISA Substrate
Substrate Type Alkaline Phosphatase
Detection Method Chemiluminescence
10

Invitrogen™ CDP-Star™ Substrate (0.25 mM Ready-To-Use) with Nitro-Block-II™ Enhancer  DFS Item

Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.

12

Invitrogen™ Luminescence Enhancer For Solution Assays, Emerald-II™ Enhancer  DFS Item

The Emerald-II chemiluminescence enhancer significantly reduces aqueous quenching and tunes the wavelength of light emission.

13

Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit

Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.

14

Thermo Scientific™ ELISA Carbonate Coating Buffer

For use as a plate coating buffer for Invitrogen Antibody Pairs or when developing a sandwich ELISA.

15

Thermo Scientific™ 1-Step™ ABTS Substrate Solution

Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.