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Western Blot Substrates and Substrate Kits

Chemiluminescent substrates and chromogenic precipitating substrates for western blot detection of alkaline phosphatase (AP) and horseradish peroxidase (HRP) enzyme conjugates; includes both routine and high-sensitivity detection materials.
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Membrane Compatibility

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1

Thermo Scientific™ 1-Step™ NBT/BCIP plus Suppressor Substrate Solution

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolyphosphate solutions for chromogenic detection of alkaline phosphatase probes.

2

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Pico PLUS + trial-size Atto

Contains SuperSignal™ West Pico PLUS Chemiluminescent Substrate plus a sample size of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate, providing chemiluminescent detection substrates for both routine and high sensitivity western blotting.

3

Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate

Recommended HRP substrate for fluorescence imagers

Contenus des kits Detection reagent 1 and 2
Type de produit ECL Western Blot Substrate
Contenu et stockage 2°C to 8°C
4

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Atto + trial-size Pico PLUS

Contains SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal™ West Pico PLUS Chemiluminescent Substrate to provide chemiluminescent detection substrates for both routine and high sensitivity western blotting.

5

Thermo Scientific™ SuperSignal™ West Femto and SuperSignal™ West Pico PLUS Sample

Provides chemiluminescent detection substrates for both routine and high sensitivity western blots

6

Thermo Scientific™ SuperSignal™ West Pico PLUS and SuperSignal™ West Femto Sample

Provides chemiluminescent detection substrates for both routine and high sensitivity western blots

7

MilliporeSigma™ Luminata Crescendo ELISA HRP Substrate

Used on immunoblots as chemiluminescent substrate

Contenu et stockage 2°C to 8°C
8

Thermo Scientific™ SuperSignal™ West Atto Ultimate Sensitivity Substrate

SuperSignal™ West Atto Ultimate Sensitivity Substrate is an enhanced chemiluminescent (ECL) substrate for high attogram protein detection and provides the high level sensitivity and better signal-to-noise ratios compared to other commercially available horseradish peroxidase (HRP) substrates.

Substrate Horseradish Peroxidase
Méthode de détection Chemiluminescence
Type de produit Substrate
Gamme de produits SuperSignal
Membrane Compatibility Nitrocellulose, PVDF
Sensibilité Low-femtogram to high-attogram
Conditions d’expédition Wet Ice
À utiliser avec (application) Western Blot
Forme Liquid
9

Thermo Scientific™ BCIP Substrate Powder (5-bromo-4-chloro-3'-indolylphosphate p-toluidine)

Powders and ready-made nitro-blue tetrazolium and 5-bromo-4-chloro-3'-indolylphosphate solutions for chromogenic detection of alkaline phosphatase probes.

10

Thermo Scientific™ SuperSignal™ Western Blot Substrate Bundle, Femto + trial-size Atto

Contains SuperSignal™ West Femto Maximum Sensitivity Chemiluminescent Substrate plus a sample size of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate to enable trial and comparison of the newest and most sensitive substrate for low abundant targets.

11

Thermo Scientific™ SuperSignal™ Western Blot Substrate Trial Pack, Atto & Pico PLUS

Contains sample sizes of SuperSignal™ West Atto Ultimate Sensitivity Chemiluminescent Substrate and SuperSignal™ West Pico PLUS Chemiluminescent Substrate to enable trial use of the most recommended substrates for routine and high-sensitivity western blotting.

13

MilliporeSigma™ Immobilon™ Block Noise Canceling Reagents

Minimize background in Western blots, increasing sensitivity for superior detection of even low-abundance proteins

Suffisant pour Nineteen 8 x 7cm miniblots
Quantité 500 mL
Contenu et stockage 20°C to 25°C
14

Thermo Scientific™ Pierce™ CN/DAB Substrate Kit

Combines chloronaphthol- and diaminobenzidine-based HRP detection for Western blot and tissue staining methods.