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Thermo Scientific™ 1-Step™ Chloronaphthol Substrate Solution

1-Step™ Chloronaphthol Substrate Solution

Supplier:  Thermo Scientific™ 34012

Catalog No. FER34012


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Description

Description

Thermo Scientific Pierce 1-Step Chloronaphthol Solution is a solution of 4-chloro-1-naphthol for the chromogenic detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.

Chloronaphthol (4-CN, MW = 178.6) reacts with HRP to form a distinct blue to blue-purple product to localize and visualize the activity of peroxidase-conjugated antibodies on blots or fixed tissue samples. The HRP substrate is not as sensitive or stable as other chromogenic substrates, such as TMB and DAB. However, the blue-purple precipitate photographs well, and its alcohol-solubility is a property that can be utilized in certain double-staining applications.

Features of Chloronaphthol Substrates:

  • HRP substrate—for detection of horseradish peroxidase activity on solid media
  • Chromogenic—no special equipment needed for visualization
  • Blue-purple—easy to photograph in color or black-and-white
  • Package options—choose powder or ready-to-use solution

    Highlights of Pierce CN Solution
  • Ready-to-use—no ethanol or methanol required to dissolve; no dilution necessary for use
  • Complete—does not require buffer or addition of hydrogen peroxide
  • Pre-filtered—does not need to be filtered before use like powders or tablets after they are dissolved
  • Stable—store refrigerated at 4°C for at least one year

    • Specifications

    Specifications

    Substrate
    Liquid
    250 mL
    Horseradish Peroxidase
    Colorimetric
    1-Step™
    Horseradish Peroxidase
    Store at 4°C.
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    For Research Use Only. Not for use in diagnostic procedures.