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  5. Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Thermo Scientific™ SuperSignal™ West Dura Extended Duration Substrate

Catalog No. PIA34075
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Catalog No. PIA34075 Supplier Thermo Scientific™ Supplier No. LSG34075
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Description

Luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

Thermo Scientific™ SuperSignal West Dura Extended Duration Substrate is a luminol-based enhanced chemiluminescence (ECL) horseradish peroxidase (HRP) substrate ideal for quantitative western blotting with stable light output for mid-femtogram level detection for western blot analysis.

SuperSignal West Dura Extended Duration Substrate for HRP is optimized for high sensitivity and long signal duration, making it ideal for cooled CCD camera-based detection systems and quantitation. Unlike substrates with signals that decline to barely detectable levels in 30–60 minutes, the signal produced with SuperSignal West Dura chemiluminescent substrate is stable for 24 hours, enabling multiple film or camera exposures.

SuperSignal West Dura Extended Duration Substrate characteristics include:

  • Sensitivity: mid-femtogram
  • Stability: 24-hr working solution stability; 1-year kit stability at room temperature
  • Compatibility: nitrocellulose and PVDF membranes
  • Signal duration: 24 hours
  • Recommended primary antibody concentration: 1:1,000–1:50,000 dilution (0.02–1.0 μg/mL)
  • Recommended secondary antibody concentration: 1:50,000–1:250,000 dilution (4–20 ng/mL)

Specifications

Product Type Substrate
Detection Method Chemiluminescence
Form Liquid
For Use With (Application) Western Blot
Membrane Compatibility Nitrocellulose, PVDF
Product Line SuperSignal
Quantity 100 mL
Substrate Horseradish Peroxidase
Recommended Antibody Concentrations 2°: 1:50-250K (4-20ng/mL), 1°: 1:5K (0.02- 1μg/mL)
Sensitivity Mid-femtogram
Signal Duration 24 hr.
Sufficient For 12 Mini-Gel Blots, 1000 sq. cm of Membrane
Kit Contents Luminol/Enhancer, 50mL, Stable Peroxide Buffer, 50mL
Content And Storage Sufficient For: 12 mini-gel blots; 1000 cm2 of membrane
• Luminol/Enhancer, 50 mL
• Stable Peroxide Buffer, 50 mL

Store at room temperature.
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For Research Use Only. Not for use in diagnostic procedures.