Flow Cytometry Compensation, Counting and Calibration

Invitrogen™ eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set

Formulated and optimized for staining with antibodies against transcription factors and nuclear proteins (e.g. Foxp3 and Ki-67), cytokines and chemokines

Invitrogen™ eBioscience™ IC Fixation Buffer

Can be used to fix cells prior to permeabilization with the Permeabilization Buffer (cat. 00-8333) when performing intracellular staining of antigens located in the cytoplasm and secretory pathway, such as cytokines, chemokines and growth factors.

Invitrogen™ eBioscience™ Permeabilization Buffer (10X)

Can be used to fix and permeabilize cells prior to performing intracellular staining of cytokines and other cytoplasmic and/or nuclear antigens.

Invitrogen™ eBioscience™ Fixation/Permeabilization Diluent

Intended to be used with antibodies to transcription factors and nuclear proteins, such as Foxp3 and Ki-67, as well as cytokines and chemokines.

Invitrogen™ BrightComp eBeads™ Compensation Bead Kits  

BrightComp eBeads compensation beads provide a consistent, accurate, and simple-to-use technique for setting flow cytometry compensation when using fluorescent protein-expressing samples.

Molecular Probes™ CountBright™ Absolute Counting Beads, for flow cytometry  

CountBright™ Absolute Counting Beads, for flow cytometry

Invitrogen™ Lysing Solution, Whole Blood Lysing Solution  

Lysing Solution, Whole Blood Lysing Solution

Molecular Probes™ Flow Cytometry Sub-micron Particle Size Reference Kit

Flow Cytometry Sub-micron Particle Size Reference Kit

Micro Particle Size Standard, Based on Polystyrene Monodisperse, Analytical Standard, Size: 0.2 m, MilliporeSigma™ Supelco™

Invitrogen™ Cell Sorting Set-up Beads (for blue lasers)  

Approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell sorting system, including laser source, optics, and stream flow.

Invitrogen™ UltraComp eBeads™ Spectral Unmixing Beads

UltraComp eBeads Spectral Unmixing Beads enhance spectral flow cytometry by providing precise single-color spectral unmixing control. Compatible with various antibodies and laser excitations, these third-generation beads offer optimized fluorophore unmixing, ensuring accurate results and superior performance.

Invitrogen™ eBioscience™ Cell Stimulation Cocktail (500X)

Cocktail of phorbol 12-myristate 13-acetate (PMA) and ionomycin. Treatment with PMA and ionomycin is sufficient to induce activation of many cell types to produce cytokines.

Invitrogen™ eBioscience™ Human Regulatory T Cell Staining Kit #2

Contains all of the buffers and monoclonal antibodies for CD4, CD25, and Foxp3 necessary to successfully stain and identify regulatory T cells from human peripheral blood cells.

Molecular Probes™ Alignflow™ Flow Cytometry Alignment Beads for Red Lasers, 2.5 μm  

Alignflow™ Flow Cytometry Alignment Beads for Red Lasers, 2.5 μm

Molecular Probes™ Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers, 2.5 μm  

Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers, 2.5 μm