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Invitrogen™ eBioscience™ Cell Stimulation Cocktail (plus protein transport inhibitors) (500X)
Can be used for induction and subsequent intracellular detection of cytokines and other secreted proteins in both in vitro-cultured and ex vivo cells
Supplier: Invitrogen™ 00497593
Description
Cocktail of phorbol 12-myristate 13-acetate (PMA), ionomycin, brefeldin A and monensin. Treatment with PMA and ionomycin is sufficient to induce activation of many cell types to produce cytokines. Brefeldin A and Monensin result in the accumulation of secreted proteins in the endoplasmic reticulum and Golgi apparatus
- A cocktail of phorbol 12-myristate 13-acetate (PMA), ionomycin, brefeldin A and monensin
- Treatment with PMA and ionomycin is sufficient to induce activation of many cell types to produce cytokines
- Brefeldin A and Monensin result in the accumulation of secreted proteins in the endoplasmic reticulum and Golgi apparatus
- Thus, it can be used for induction and subsequent intracellular detection of cytokines and other secreted proteins in both in vitro-cultured and ex vivo cells
Specifications
| 100 μL | |
| Flow Cytometry, Functional Assays, Cell Culture | |
| eBioscience | |
| Cell Stimulation Cocktail |
For Research Use Only. Not for use in diagnostic procedures.