Crosslinking Reagents
Invitrogen™ Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP)
The succinimidyl ester chemically reacts with primary amines and after mild reduction of the dithiol, it can react form a disulfide with other thiols.
Thermo Scientific™ NHS-Phosphine
Derivatize proteins or amine surfaces for selective conjugation, detection or purification of azide-tagged targets with these activated phosphine compounds.
Thermo Scientific™ Sulfo-SIAB (sulfosuccinimidyl (4-iodoacetyl)aminobenzoate)
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker composed of sulfo-NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 9-atom spacer arm.
Thermo Scientific™ Sulfo-LC-SDA (Sulfo-NHS-LC-Diazirine) (sulfosuccinimidyl 6-(4,4'-azipentanamido)hexanoate)
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).
Thermo Scientific™ Sulfo-SMPB (sulfosuccinimidyl 4-(N-maleimidophenyl)butyrate)
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of an aromatic, 10-atom spacer arm.
Thermo Scientific™ Sulfo-MBS (m-maleimidobenzoyl-N-hydroxysulfosuccinimide ester)
Crosslink amines to sulfhydryls with this short, water-soluble crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of an aromatic, 6-atom (7.3A) spacer arm.
Thermo Scientific™ Sulfo-GMBS (N-γ-maleimidobutyryl-oxysulfosuccinimide ester)
Crosslink amines to sulfhydryls with this short, water-soluble crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of an aliphatic, 6-atom (7.3A) spacer arm.
Thermo Scientific™ PEG4-SPDP (PEGylated, long-chain SPDP crosslinker)
Long (25.7A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 4-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.
Thermo Scientific™ KMUH (N-κ-maleimidoundecanoic acid hydrazide)
Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this long (19.0A) crosslinker containing maleimide and hydrazide groups.
Thermo Scientific™ SBAP (succinimidyl 3-(bromoacetamido)propionate)
Crosslink amines to sulfhydryls with this short crosslinker composed of NHS-ester and bromoacetyl (haloacetyl) groups at ends of a 7-atom (6.2A) spacer arm.
Thermo Scientific™ DST (disuccinimidyl tartrate)
Crosslink amines to amines with disuccinimidyl tartrate, composed of NHS-ester groups and a diol-containing spacer arm (6.4A) that is periodate-cleavable.
Thermo Scientific™ DTBP (Wang and Richard's Reagent)
Crosslink amines to amines with dithiobispropionimidate; composed of imidoester groups at either end of a reducible (cleavable) disulfide-containing spacer arm.
Thermo Scientific™ Sulfo-KMUS (N-κ-maleimidoundecanoyl-oxysulfosuccinimide ester)
Crosslink amines to sulfhydryls with this long crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of an aliphatic, 13-atom (16.3A) spacer arm.
Thermo Scientific™ BS3-d4 (bis(sulfosuccinimidyl) 2,2,7,7-suberate-d4)
Crosslink amines to amines using these heavy (deuterated) and light matched pairs of an 11.4A, NHS-ester crosslinker for mass spectrometry analysis of protein interactions.
Thermo Scientific™ EGS (ethylene glycol bis(succinimidyl succinate))
Crosslink amines to amines with this membrane permeable crosslinker composed of NHS-ester groups and a 16.1A hydroxylamine-cleavable spacer arm.