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Thermo Scientific™ Pierce™ NHS-Azide Staudinger Ligation Reagents

Activated azido compounds to derivatize proteins or amine surfaces for selective conjugation, detection or purification by phosphine-modified probes.

Manufacturer: thermo scientific™  88902

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Catalog No. PI88902


Description & Specifications


Chemical Reactivity Amine-to-Nonselective
Cleavable No
Description Pierce NHS-Azide
Formulation Ethane functionalized with succinimidyl and azido ends; solid
Membrane Permeable Yes
Molecular Weight 198.14
PEGylated No
Quantity 10mg
Sufficient For 50mL of typical 1X (1mM) reaction solution, or approx. 5 uses at 2mg reagent per use
Spacer Arm Length 2.5Å
Water Soluble No

Thermo Scientific™ Pierce NHS-Azide reagents are amine-reactive compounds that can be used to derivatize primary amines of proteins or amine-coated polymer surfaces for ligation to phosphine-modified molecules.

These NHS-ester compounds react to form covalent bonds with primary amines (e.g., side chain of lysine residues or aminosilane-coated surfaces). The azide (N3) group reacts with phosphine-labeled molecules by a mechanism known as Staudinger chemistry, enabling efficient and specific conjugation of derivatized molecules in biological samples. In combination with different phosphine-activated compounds, these NHS-Azide reagents facilitate a variety of labeling and crosslinking experimental strategies for investigating protein interactions and cellular pathways.


  • Soluble – reagents easily dissolve in water-miscible solvents for subsequent dilution in aqueous reaction mixtures with cell lysates and other biological samples

  • Compatible – reaction chemistry occurs effectively in simple buffer conditions; requires no accessory reagents such as copper or reducing agents

  • Specific – NHS esters are specific for covalent attachment to primary amines (side chain of lysine or N-terminus of polypeptides)


  • Chemoselective – azide and phosphine groups do not react or interfere with components of biological samples but conjugate to one another with high efficiency

  • Pegylated – choose the very short NHS-Azide or one of the two varieties whose spacer arms contain medium (4-unit) or long (12-unit) polyethylene glycol (PEG) groups for increased solubility and reach