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UltraPure Guanidine Hydrochloride is a strong protein denaturant. In a highly concentrated (5 to 6M) guanidine hydrochloride solution, many proteins unfold and separate into their constituent polypeptide chains.
Ensure uniform lot-to-lot consistency in preparing electrophoresis gels using highly purified and blended regular- or low-melting agarose formulations.
Perform DNA extractions with this phenol:chloroform:isoamyl alcohol mixture (25:24:1), accompanied by a buffer to equilibrate the solution at either pH 6.7 or pH 8.0.
Invitrogen Yeast tRNA purified from brewer's yeast. When used at a final concentration of 10 to 20 μg/mL, yeast tRNA is an effective coprecipitant to aid in recovery of small amounts of nucleic acids.
Invitrogen Molecular biology grade, 5 M Ammonium Acetate solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.
Invitrogen Molecular biology grade, 0.5 M EDTA, pH 8.0 solution is supplied in one bottle. The solution is certified RNase-free, economical, and ready-to-use.
Invitrogen IPTG (Isopropyl-beta-D-thiogalactoside) is an inducer of the lac operon and is frequently used in conjunction with X-GAL to determine the lac phenotype of recombinant plasmids or phage.
Invitrogen Linear Acrylamide (5 mg/mL) is for use as a nucleic acid coprecipitant and is ideal for PCR and RT-PCR reactions since small amounts of contaminating nucleic acids present in other carriers could be amplified.
Invitrogen molecular biology grade, 2M KCl solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.
In RNA extraction procedures, Acid Phenol:Chloroform:IAA aids in the removal of DNA (it partitions into the organic phase), helps to stabilize the interface, and prevents foaming when mixing.