Miscellaneous Molecular Biology Reagents
Invitrogen™ IPTG
IPTG (isopropylthio-β-galactoside), a structural non-metabolizable analogue of allolactose, is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-Gal or Bluo-Gal to detect lac gene activity in cloning procedures.
Invitrogen™ X-Gal
X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays.
MilliporeSigma™ Calbiochem™ α-D-Glucose-6-phosphate, Monosodium Salt
Aqueous stock solutions are stable for up to 6 weeks at -20°C
MilliporeSigma™ Calbiochem™ FURA 2/AM
Cell-permeable ester form of FURA 2
MilliporeSigma™ Calbiochem™ D-Galactose
Aldo hexose
Thermo Scientific™ IPTG Solution, ready-to-use
Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.
Thermo Scientific™ Yeast DNA Extraction Kit
Quickly, efficiently and easily lyse yeast cells to release DNA with Y-PER lysis reagent.
MilliporeSigma™ Calbiochem™ FURA 2/AM
1 mM solution of FURA 2/AM
Thermo Scientific™ X-Gal
Effectively screen colonies with X-Gal, an inert chromogenic substrate for beta-galactosidase which hydrolyzes X-Gal, forming an intense blue precipitate.
MilliporeSigma™ Calbiochem™ DNase I, Bovine Pancreas
Acts on single and double stranded DNA
Poloxamer 188
Formule moléculaire: C5H10O2 Synonyme: Pluronic™ F-68; Polyoxyethylene-polyoxypropylene block copolymer
Invitrogen™ dNTP Mix (2.5 mM)
The same high-quality deoxynucleotide triphosphate solutions provided in the Invitrogen™ RETROscript™ First Strand cDNA Synthesis Kit for RT-PCR.
Invitrogen™ PureLink™ RNase A (20 mg/mL)
PureLink™ RNase A (ribonuclease A) is a bovine pancreatic ribonuclease that cleaves single-stranded RNA.
Thermo Scientific™ DTT (dithiothreitol)
Stabilize enzymes and other proteins which possess free sulfhydryl groups with DTT, shown to restore activity lost by oxidation of these groups in vitro.