Miscellaneous Molecular Biology Reagents
Invitrogen™ Guanidine Isothiocyanate
UltraPure Guanidine Isothiocyanate is a strong protein denaturant when used at high concentrations.
Pharmalytes™ pH range 5.0 - 8.0, MilliporeSigma™ Supelco™
Pharmalytes™ are ampholytes which are used to create a pH gradient under application of an electric field.
Pharmalytes™ pH range 4.0 - 6.5, MilliporeSigma™ Supelco™
Pharmalytes™ are ampholytes which are used to create a pH gradient under application of an electric field.
Invitrogen™ UltraPure™ Buffer-Saturated Phenol
UltraPure™ Buffer-Saturated Phenol is used in the purification of nucleic acids. The reagent, which consists of UltraPure™ Phenol that has been saturated with Tris-HCl buffer, is already buffer equilibrated to pH >7.4.
Invitrogen™ Salmon Sperm DNA, sheared (10 mg/mL)
Sheared Salmon Sperm DNA is a very effective blocking agent when used in Northern prehybridization and hybridization buffers at a concentration of 100 to 200 μg/mL.
MilliporeSigma™ Calbiochem™ FURA 2/AM
Cell-permeable ester form of FURA 2
Invitrogen™ MagMAX™ Wash Solution 2 Concentrate
Invitrogen MagMAX Wash Solution 2 Concentrate is a component of all the MagMAX Isolation Kits that may be purchased separately.
MilliporeSigma™ Calbiochem™ Hypoxanthine
Used in conjunction with thymidine and aminopterin for cloning and selection of hybridoma cell lines
Invitrogen™ UltraPure™ Guanidine Hydrochloride
UltraPure Guanidine Hydrochloride is a strong protein denaturant. In a highly concentrated (5 to 6M) guanidine hydrochloride solution, many proteins unfold and separate into their constituent polypeptide chains.
Pharmalytes™ pH range 2.5 - 5.0, MilliporeSigma™ Supelco™
Pharmalytes™ are ampholytes which are used to create a pH gradient under application of an electric field.
Invitrogen™ ATP Solution (10 mM)
This Invitrogen ribonucleotide triphosphate (rNTP) solution is a component of the MAXIscript™ Kits and is supplied in one tube containing 100 μL.
Thermo Scientific™ IPTG, dioxane-free
Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.
Thermo Scientific™ X-Gluc
Effectively screen colonies with X-Gluc, a substrate for ß-glucuronidase (GUS) which is encoded by gusA, a widely used reporter gene.
Thermo Scientific™ EDTA
Chelate alkaline earth metals. Thermo Scientific™ Pierce EDTA is highly purified for use in a variety of laboratory methods.
Invitrogen™ Formamide (Deionized)
Ambion formamide has been deionized, is nuclease-free, and comes packaged as a liquid in an amber glass bottle under argon.