missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Molecular Biology Reagents and Kits
  4. Miscellaneous Molecular Biology Reagents
  5. FERR1171

Thermo Scientific™ IPTG Solution, ready-to-use

Catalog No. FERR1171
Change view
Click to view available options
Quantity:
10 x 1.5 mL
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
FERR1171 10 x 1.5 mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. FERR1171 Supplier Thermo Scientific™ Supplier No. R1171

Description

Induce the expression of genes under the control of lac operon in cloning and protein expression procedures.

IPTG (isopropyl-beta-D-thiogalactopyranoside) is a highly stable synthetic analog of lactose. It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon . It is used in conjunction with X-Gal (#R0941) to determine the lac phenotype in blue/white colony screening.

It inactivates the lac repressor and induces synthesis of beta-galactosidase, an enzyme that promotes lactose utilization. IPTG is used to induce expression of cloned genes under control of the lac operon. It is used in conjunction with X-Gal to determine the lac phenotype in blue/white colony screening.

Ready-to-use 100mM IPTG aqueous solution is non-toxic, stable, 0.22μm membrane-filtered, formulated for direct use in blue/white colony screening.

Specifications

Chemical Name or Material Isopropylthiogalactoside
Concentration 0.1 M
For Use With (Application) Protein Expression
Product Type IPTG Solution
Quantity 10 x 1.5 mL
Form Liquid

Frequently Asked Questions (FAQs)

How do I resuspend IPTG and X-Gal?

IPTG can be reconstituted in water. Make a stock of 100 mM in water and store working aliquots at -20°C. X-gal can be reconstituted in DMSO, or in a 50:50 mix of DMSO and water. To do the latter, you must dissolve in DMSO first, and then add water to bring up to final volume. It is not necessary to filter sterilize these solutions.

The X-gal solution should be protected from light. To make plates, add 50 ug/ml X-gal and 1 mM (0.24 mg/mL) IPTG to LB/agar that has been cooled down to 50°C. To spread on top of plates, use 50 µl 2% stock of X-gal and 30 µl 100 mM stock of IPTG. 

For Research Use Only. Not for use in diagnostic procedures.