Cell Based Assays
MilliporeSigma™ Calbiochem™ Caspase-1 Assay Kit, Colorimetric
Measures activity of caspase-1 and related caspases that recognize sequence YVAD
Invitrogen™ DASPEI (2-(4-(dimethylamino)styryl) -N-Ethylpyridinium Iodide)
Stains mitochondria of live cells
Invitrogen™ SYTO™ 62 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO
For use with fluorescence microscopy and flow cytometry
Invitrogen™ SYTO™ 64 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO
For use with fluorescence microscopy and flow cytometry
Invitrogen™ SYTO™ Blue Fluorescent Nucleic Acid Stain Sampler Kit (SYTO™ dyes 40, 41, 42, 45)
Includes cell-permeant, blue-fluorescent SYTO dyes
Invitrogen™ SYTO™ 83 Orange Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO
For use with fluorescence microscopy and flow cytometry
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 450
A viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation, and/or permeabilization procedures.
Invitrogen™ Dextran, Tetramethylrhodamine, 70,000 MW, Neutral
Rigorous methods for removing as much unconjugated dye as practical, followed by an assay of dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants
MilliporeSigma™ Cysteine Sulfenic Acid Probe (DCP-Bio1)
DCP-Bio1, which consists of the sulfenic acid-reactive 3-(2,4-dioxocyclohexyl) propyl (DCP) appended to biotin, is a useful reagent for characterizing cellular cysteine sulfenic acid
Invitrogen™ eBioscience™ BrdU Staining Buffer Set for Flow Cytometry
Contains the buffers needed for optimal BrdU staining with the BU20A monoclonal antibody for flow cytometric analysis. This set, in combination with BrdU and DNase I steps, allows for detection of BrdU pulse-labeled cells, thereby enabling the examination of proliferating cells.
MilliporeSigma™ PI3 Kinase Activity/Inhibitor ELISA
Used to measure & quantify PI 3-Kinase levels
Molecular Probes™ FluxOR™ Potassium Ion Channel Assay
FluxOR™ Potassium Ion Channel Assay
Invitrogen™ Fluo-5N, AM, cell permeant - Special Packaging
Analog of fluo-4 with lower Ca2+-binding affinity, making it suitable for detecting intracellular calcium levels in 1μM to 1mM range that would saturate response of fluo-3 and fluo-4
Invitrogen™ Dextran, Texas Red™, 3000 MW, Lysine Fixable
Rigorous methods for removing as much unconjugated dye as practical, followed by assay of dextran conjugates by thin-layer chromatography to help ensure absence of low molecular weight contaminants
MilliporeSigma™ Chemicon™ QCM™ Tumor Cell Transendothelial Migration Assay
Analyzing ability of leukocytes to migrate through endothelium