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PCR Cloning Kits

Chemical reagents, kits, and other products used for polymerase chain reaction (PCR) cloning.
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1

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with TOP10F' E. coli  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning

Souche bactérienne ou de levures TOP10F´
Format Kit
Type de produit Cloning Kit
Vecteur TOPO-TA Cloning Vectors
Gamme de produits One Shot
Méthode de clonage TOPO™-TA
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
2

Invitrogen™ GeneRacer™ Kit with AMV RT and TOPO TA Cloning™ Kit for Sequencing  DFS Item

Advanced RACE (rapid amplification of cDNA ends) technique that improves efficiency of amplifying full-length 5' and 3' cDNA ends

3

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli  DFS Item

Provides a highly efficient, 5 minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing

Souche bactérienne ou de levures DH5α
Format Kit
Type de produit Cloning Kit
Vecteur TOPO-TA Cloning Vectors
Gamme de produits One Shot
Accélérateur T7, T3
Méthode de clonage TOPO™-TA
À utiliser avec (application) Chromatin Biology
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™4-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• T3, T7, M13 forward, and M13 reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
4

Invitrogen™ pLenti6/V5 Directional TOPO™ Cloning Kit  DFS Item

Directional TOPO Cloning site for rapid and efficient directional cloning of blunt-end PCR products

5

Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit  DFS Item

Used in suspension-adapted cells for transient protein expression. Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.

6

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 chemically competent E. coli cells  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning

Souche bactérienne ou de levures TOP10
Format Kit
Type de produit Cloning Kit
Type de cellule Chemically Competent
Méthode de clonage TOPO™-TA
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™2.1-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid

Store at -68 to -85°C.
7

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with pCR™4-TOPO™ Vector, One Shot™ TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit  DFS Item

Provides a 5 minute, one-step cloning strategy. Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with pCR4-TOPO Vector, One Shot™ TOP10 Chemically Competent E. coli and PureLink Plasmid Prep Kit enables direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing.

9

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, with pCR™II-TOPO™ Vector and One Shot™ TOP10 Electrocomp™ E. coli  DFS Item

Designed for cloning PCR products directly from a PCR reaction in just 5 minutes

Souche bactérienne ou de levures TOP10
Format Kit
Type de produit Cloning Kit
Vecteur TOPO-TA Cloning Vectors
Gamme de produits One Shot
Accélérateur T7, SP6
Méthode de clonage TOPO™-TA
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.

Box 2:
• One Shot™ Chemically Competent or Electrocomp™ E. coli
• S.O.C. medium
• Supercoiled pUC19 control plasmid.

Store at -68 to -85°C.
10
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Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing

11

Invitrogen™ TOPO™ TA Cloning™ Kit, Dual Promoter, without competent cells  DFS Item

Résistance aux antibiotiques des bactéries Ampicillin (AmpR), Kanamycin (KanR)
Format Kit
Vecteur TOPO-TA Vectors
Gamme de produits TOPO
Accélérateur T7, SP6
Méthode de clonage TOPO™-TA
Contenu et stockage Box 1:
• Topoisomerase I-activated pCR™II-TOPO™ vector
• PCR buffer
• Salt solution
• dNTPs
• Control template
• M13 forward and reverse primers
• Control PCR primers
• Sterile water

Store at -5 to -30°C.
All reagents are stable for 6 months when properly stored.
12

Invitrogen™ GeneRacer™ Kit with SuperScript™ III RT and Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing  DFS Item

Advanced RACE (rapid amplification of cDNA ends) technique that improves efficiency of amplifying full-length 5' and 3' cDNA ends

13

Invitrogen™ GeneRacer™ Kit with SuperScript™ III RT and TOPO TA Cloning™ Kit for Sequencing  DFS Item

Ensures amplification of only full-length transcripts via elimination of truncated messages from amplification process

14

Invitrogen™ Ambion™ T4 RNA Ligase, cloned, 5 U/μL  DFS Item

Catalyzes formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor

15

Invitrogen™ RNase T1 (1 U/μL)  DFS Item

Invitrogen™ RNase T1, RNA-Grade cleaves 3' of single-stranded G residues and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites.