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DNA Cloning and Mapping and Mutagenesis

Chemical reagents, kits, and other products used for DNA cloning and mapping, and to carry out the process of mutagenesis.
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1

Thermo Scientific™ Lambda DNA (dam–, dcm–)

Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.

2

Thermo Scientific™ Lambda DNA

Generate DNA size marker fragments with this linear, double-stranded DNA, a common substrate for restriction endonucleases.

3

Thermo Scientific™ pUC19 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.

4

Thermo Scientific™ pUC18 DNA

Optimize cloning with this double-stranded closed circular high copy plasmid DNA, 2686 base pairs with a molecular weight of 1.74 x 106 Da isolated from E.coli.

5

Thermo Scientific™ aLICator LIC Cloning and Expression Kit 1 (untagged)

Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.

6

Invitrogen™ GeneArt™ Gibson Assembly EX Master Mix  DFS Item

GeneArt Gibson Assembly EX Master Mix enables DNA assembly via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a single-tube, two-step optimized reaction.

Type de contrôle Positive Control
Type de produit Master Mix
Gamme de produits GENEART
Nombre de fragments Up to 15 fragments
Étape du flux de travail DNA Assembly
Conditions d’expédition Dry Ice
Méthode de clonage Seamless cloning
À utiliser avec (application) Seamless Cloning & Genetic Assembly
Contenu et stockage Includes master mix, positive control, and water and accommodates the use of your own competent cells.
7

Invitrogen™ TrueTag™ Donor DNA Kit, DDK  DFS Item

The Invitrogen TrueTag Donor DNA Kit, DDK, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

8

Invitrogen™ TrueTag™ Donor DNA Kit, Myc  DFS Item

The Invitrogen TrueTag Donor DNA Kit, Myc, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

9

Invitrogen™ GeneArt™ Gibson Assembly HiFi Cloning Kit, chemically competent cells  DFS Item

The GeneArt Gibson Assembly HiFi Cloning Kit enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

10

Invitrogen™ GeneArt™ Gibson Assembly HiFi Master Mix  DFS Item

GeneArt Gibson Assembly HiFi Master Mix enables DNA assembly and cloning via a technique that allows multiple overlapping DNA fragments to be seamlessly linked in a one-step, 15–60 minute isothermal reaction.

Type de contrôle Positive Control
Type de produit HiFi Master Mix
Gamme de produits GENEART
Nombre de fragments Up to 6 fragments
Étape du flux de travail DNA Assembly
Conditions d’expédition Ambient, Wet Ice, Dry Ice
Méthode de clonage Seamless cloning
À utiliser avec (application) Seamless Cloning & Genetic Assembly
Contenu et stockage Includes master mix, positive control, and water and accommodates the use of your own competent cells.
11

Invitrogen™ TrueTag™ Donor DNA Kit, GFP  DFS Item

The Invitrogen™ TrueTag™ Donor DNA Kit, GFP, provides a rapid solution to drive high-efficiency gene tagging (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.

12

Thermo Scientific™ Fast DNA End Repair Kit

Perform blunting and phosphorylation of DNA ends in just 5 minutes for subsequent use in blunt-end ligation with this kit.

13

Thermo Scientific™ aLICator LIC Cloning and Expression Kit 4 (N-terminal His-tag/WQ)

Get fast and efficient ligation-independent cloning and tight regulation of gene expression in E. coli. with this cloning and expression system.

14

Thermo Scientific™ phiX174 RF1 DNA

Prepare DNA molecular weight standards with Thermo Scientific™ phiX174 RF1 DNA, a 5386 bp double-stranded closed circular plasmid.

15

Invitrogen™ TrueTag™ Knockout Enrichment Donor DNA Kit  DFS Item

The Invitrogen TrueTag Knockout Enrichment Donor DNA Kit provides a rapid solution to drive high-efficiency gene knockout and enrichment (up to 100% edited cells) with transfection-ready donor DNA for genome editing experiments.