Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (20 mL sample prep, 10 mL PCR)
Go from Biological Sample to Genotyping Results in less than one hour.
Applied Biosystems™ resDNASEQ™ dPCR E. coli DNA Kit
The Applied Biosystems resDNASEQ dPCR E. coli DNA Kit is a digital PCR (dPCR)-based solution to detect host-cell DNA from E. coli, an expression system commonly used for production of recombinant proteins and plasmids.
Applied Biosystems™ ViralSEQ™ Quantitative Sf-Rhabdovirus Kits
The Applied Biosystems ViralSEQ Quantitative Sf-Rhabdovirus Kit is a two-step RT-qPCR assay for the detection of Sf-rhabdovirus in recombinant products manufactured in insect cell lines using baculovirus expression systems.
Applied Biosystems™ Megaplex™ RT Primers, Human Pool B v3.0
Megaplex™ RT Primers, Human Pool B v3.0
Applied Biosystems™ resDNASEQ™ Quantitative Vero DNA Kits
The resDNASEQ Quantitative Vero DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Vero (African Green Monkey) cells, an expression system commonly used for the production of vaccines.
Applied Biosystems™ resDNASEQ™ Quantitative NS0 DNA Kits
The resDNASEQ™ Quantitative NS0 DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from NS0 (murine myeloma) cells, an expression system commonly used for the production of vaccines.
Invitrogen™ EXPRESS One-Step Superscript™ qRT-PCR Kit, universal
Kit incorporates proven Invitrogen™ enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for probe-based qPCR experiments.
Applied Biosystems™ Megaplex™ Primer Pools, Rodent Pools Set v3.0
Megaplex™ Primer Pools, Rodent Pools Set v3.0 is available under a single part number containing both the Megaplex™ RT Primers and Megaplex™ PreAmp Primers, Rodent Pools Sets.
Applied Biosystems™ TaqMan™ Advanced miRNA cDNA Synthesis Kit
Kit uses 3' poly-A tailing and 5' ligation of an adaptor sequence to extend the mature miRNAs present in the sample on each end prior to reverse transcription.
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Applied Biosystems™ MycoSEQ™ Mycoplasma Detection Kits
The MycoSEQ Mycoplasma Detection Kit is a real-time mycoplasma PCR assay designed and validated to meet the requirements of European Pharmacopeia Chapter 2.6.7 for use as a nucleic acid amplification technique (NAT).
Applied Biosystems™ resDNASEQ™ Quantitative CHO DNA Kit, 100 reactions
This quantitative PCR (qPCR)-based kit, part of the resDNASEQ™ Quantitative CHO DNA System, allows users to detect sub-picogram amounts of residual DNA from CHO cell line in under 5 hours.
Applied Biosystems™ resDNASEQ™ Quantitative MDCK DNA Kits
The resDNASEQ™ Quantitative MDCK DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from MDCK (canine kidney) cells, an expression system commonly used for the production of vaccines.
Applied Biosystems™ Path-ID™ Multiplex One-Step RT-PCR Kit
The Path-ID™ Multiplex One-Step RT-PCR Kit is designed for multiplex, quantitative, reverse transcription PCR (qRT-PCR).
Applied Biosystems™ TaqMan™ Bacillus anthracis Detection Kit
TaqMan™ Bacillus anthracis Detection Kit