Real Time PCR Reagents and Kits
Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
Applied Biosystems™ TaqMan™ RNA-to-CT™ 1-Step Kit
Delivers consistent RNA target quantification for a wide variety of assays and is validated with Applied Biosystems™ comprehensive library of TaqMan™ Gene Expression assays.
Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents
AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.
Applied Biosystems™ TaqMan™ Fast Reagent Starter Kit
Designed to detect and quantify Eukaryotic 18S rRNA sequence in cDNA samples using the TaqMan 5' nuclease assay, in fast thermal cycling mode.
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Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
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Invitrogen™ SuperScript™ III Platinum™ One-Step qRT-PCR Kit
Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.
Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents
TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe.
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Invitrogen™ ROX Reference Dye
ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR.
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Thermo Scientific™ ABsolute™ Probe-Based Detection qPCR Master Mixes
Thermo Scientific™ ABsolute qPCR Master Mixes offer sensitivity and reproducibility for real-time PCR applications.
Applied Biosystems™ TaqCheck™ SARS-CoV-2 Control
The Applied Biosystems™ TaqCheck™ SARS-CoV-2 Control is a synthetic RNA positive control that contains target sequences for the SARS-CoV-2 S and N gene assays included in the TaqCheck™ SARS-CoV-2 Fast PCR Assay.
VetMAX™ PEDV/TGEV/SDCoV Kit
A single-well multiplex real-time RT-PCR kit for the detection and differentiation of the porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and swine delta corona virus (SDCoV) in environmental samples.
Applied Biosystems™ VetMAX™ PRRSV 3.0 Reagents
VetMAX™ PRRSV 3.0 Reagents contain master mix, Xeno RNA control, and a primers/probes mix that enables multiplex, one-step, reverse transcription and real-time PCR (RT-PCR) assays targeting North American (NA) and European (EU) porcine reproductive and respiratory syndrome virus (PRSSV) RNA and Xeno RNA.
Applied Biosystems™ QuantStudio™ Absolute Q™ Digital PCR Starter Kit
The QuantStudio Absolute Q Digital PCR Starter Kit is designed to confirm the performance of the QuantStudio Absolute Q Digital PCR System either at the time of system installation or as part of future, user-defined system verification procedures.
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Applied Biosystems™ TaqMan™ QSY and QSY2 Probes
Applied Biosystems TaqMan QSY and QSY2 probes are dual-labeled probes used for real-time PCR. TaqMan QSY and QSY2 Probes are designed for optimal sensitivity and performance in multiplexed qPCR experiments for detection of up to 6 targets.
Applied Biosystems™ PureQuant B Cell Assay
The Applied Biosystems PureQuant assays are a family of assays that accurately identify and quantify specific immune cell types in a mixed population of cells. These assays are TaqMan qPCR-based and measure genomic DNA methylation at specific sites that serve as a unique identifier. The assays include standards and controls and each 96-well plate can accommodate up to seven test samples.