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General Western Blot Reagents

Specially formulated reagents for a variety of analytical western blot processes; includes chromogenic and chemiluminescent kits and substrates, transfer solutions, enhancing buffers, and stripping buffers for reprobing under alternate conditions.
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1 – 15 of 45 results
1

Invitrogen™ Novex™ Tris-Glycine SDS Buffer Kit  DFS Item

Made with high-purity reagents and are strictly quality controlled

2

Invitrogen™ Luminescence Enhancer For Membrane Blotting Assays, Nitro-Block-II™ Enhancer  DFS Item

Use with CSPD™ or CDP-Star™ chemiluminescent substrates to increase the alkaline phosphatase signal for protein or nucleic acid blots. The Nitro-Block-II membrane enhancer increases chemiluminescent signal intensity on nitrocellulose and PVDF

3

Thermo Scientific™ Restore™ Fluorescent Western Blot Stripping Buffer

Quickly and gently remove primary and near-infrared (IR) dye-labeled secondary antibodies from Western blots.

Content And Storage Upon receipt store at room temperature.
Form Liquid
Product Line Restore
5

Thermo Scientific™ SuperSignal™ Western Blot Enhancer

Increase both signal intensity and sensitivity 3- to 10-fold with this membrane treatment reagent and primary antibody diluent.

Content And Storage Upon receipt store solutions at 4°C Products are shipped at ambient temperature.
Form Liquid
Product Type Western Blot Enhancer
Membrane Compatibility Nitrocellulose, PVDF
For Use With (Application) Western Blot
Product Line SuperSignal
Detection Method Chemiluminescence, Fluorescence, Colorimetric
6

Invitrogen™ WesternBreeze™ Chromogenic Kit, anti-mouse  DFS Item

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

7

Invitrogen™ WesternBreeze™ Blocker/Diluent (Part A and B)  DFS Item

Optimized, easy-to-use blocker and primary antibody diluent system

8

Invitrogen™ WesternBreeze™ Chemiluminescent Kit, anti-rabbit  DFS Item

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

9

Invitrogen™ WesternBreeze™ Chromogenic Kit, anti-goat  DFS Item

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

10

Thermo Scientific™ Pierce™ 1-Step Transfer Buffer

Perform rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.

Product Type Transfer Buffer
11

Invitrogen™ WesternBreeze™ Wash Solution (16X)  DFS Item

Optimized solution of concentrated buffered saline containing detergent

12

Invitrogen™ WesternBreeze™ Chromogenic Kit, anti-rabbit  DFS Item

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

13

Invitrogen™ WesternBreeze™ Chemiluminescent Kit, anti-mouse  DFS Item

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

14

Invitrogen™ WesternBreeze™ Chemiluminescent Kit, anti-goat  DFS Item

Detect proteins that have been immobilized on membranes (nitrocellulose or PVDF) following western transfer or bound directly from solution (dot blots)

15

Invitrogen™ No-Stain™ Protein Labeling Reagent  DFS Item

A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.

Shipping Condition Dry Ice
Description The No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins
Product Type Protein Labeling Reagent
For Use With (Equipment) Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager
Membrane Compatibility Nitrocellulose, PVDF
Quantity 1 kit
Shelf Life At least 2 years from receipt
Run Time 10 minutes
For Use With (Application) Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection
Gel Compatibility Any Gel Type and Gel Chemistry
Product Line Invitrogen