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Invitrogen™ Bolt™ Antioxidant

Catalog No. BT0005
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BT0005 15 ml
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Catalog No. BT0005 Supplier Invitrogen™ Supplier No. BT0005
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Description

Requires

A Bolt™ Mini Gel Tank is required for use of all Bolt gels.

A proprietary reagent that maintains proteins in a reduced state during protein gel electrophoresis

Bolt Antioxidant migrates with reduced proteins to maintain reducing conditions and to prevent re-oxidation of sensitive amino acids such as methionine and tryptophan. Bolt Antioxidant must be used with samples reduced using Bolt Sample Reducing Agent or other reducing agent.

  • Optimized for neutral pH of Bolt Bis-Tris Plus gels and is not compatible with other gel chemistries
  • Can also be used with Bolt Transfer Buffer to enhance transfer of reduced proteins to membranes when using Mini Blot Module

1D Gel Electrophoresis, Protein Gel Electrophoresis, Proteins, Expression, Isolation and Analysis, Western Blotting

Specifications

Product Line Bolt
Gel Compatibility Bolt Bis-Tris Plus Gel
Product Type Antioxidant
Buffer Antioxidant
Form Liquid
Content And Storage Store at 4°C.
Quantity 15 ml

Frequently Asked Questions (FAQs)

Can I prepare my protein sample with the reducing agent and store it for future use?

DTT is not stable, so it must be added and the reduction performed just prior to loading your samples.
My LDS or SDS sample buffer precipitates when stored at 4 degrees C. Can I warm it up? Can I store it at room temperature?

Precipitation of the LDS or SDS at 4 degrees C is normal. Bring the buffer to room temperature and mix until the LDS/SDS goes into solution. If you do not want to wait for it to dissolve, you can store the sample buffer at room temperature.
What are the storage conditions for Bolt gels?

Similar to NuPAGE gels with storage temperatures of 4 to 25 degrees C.
What is the shelf life for Bolt gels?

The shelf life for Bolt gels is similar to that for NuPAGE gels: 12 months from the date of shipment.
How are Bolt gels different than NuPAGE gels?

While they are both Bis-Tris based gels, the chemistries are very different since Bolt gels are optimized for western blotting. Another key difference is the wedge well design of the Bolt gels, which allows larger sample volumes to be loaded.
Which power supplies are compatible with the Mini Gel Tank?

The most common power supplies from Thermo Fisher Scientific, Bio-Rad, and Hoefer are compatible. Also, power supply adapters are available for power supplies not designed for use with covered or non-retractable power leads. Thermo Fisher Owl branded gel tanks are not compatible
Are Bolt gels compatible with transfer devices from other suppliers?

Yes. While we would prefer that you use our devices, Bolt gels can also be transferred using devices from Bio-Rad, including the Mini Trans-Blot Cell, Trans-Blot SD Semi-Dry Transfer Cell, or Trans-Blot Turbo Transfer System.
Are Bolt gels compatible with other transfer devices?

Yes. You can use the iBlot Dry Transfer System or XCell II Blot Module with SureLock tank and achieve similar transfer efficiency.

For Research Use Only. Not for use in diagnostic procedures.