HPLC Guard Columns and Cartridges
MilliporeSigma™ Supelco™ Zenix™ SEC-100 Gel Filtration Guard Column, 3 μm Particle Size, Stainless Steel
Like their 5 micron siblings, 3 micron Zenix™ SEC columns feature a hydrophilic surface layer to prevent the sample from interacting with the underlying silica.
MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C5 Supelguard™ Cartridge, 5 μm Particle Size
Kits include one cartridge, a stand-alone holder, a piece of tubing, and 2 nuts and ferrules. Guard cartridges require holders that are sold separately. The 2.1 and 4 mm I.D. cartridges use 21150AST or 59660-U (both stand-alone) or 504254 or 55205 (both integral). The 10 mm I.D. cartridges use 567499-U. The 21.2 mm I.D. cartridges use 581392-U.
MilliporeSigma™ Supelco™ Astec™ Cellulose DMP Chiral HPLC Guard Kit, 5 μm Particle Size
Guard cartridges to protect Astec™ Cellulose DMP columns
MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-Si Supelguard Cartridge Kit, 5 μm Particle Size
A typical mobile phase used with normal-phase silica columns consists of a hydrocarbon solvent such as hexane or heptane, mixed with a relatively small percentage of more polar solvent.
MilliporeSigma™ Supelco™ Proteomix™ WCX-NP3 Weak Cation Exchange Guard Cartridge and Holder, 3 μm Particle Size, Stainless Steel
Used for the analysis or small scale purification of positively charged biopolymers including glycoprotein isoforms and mAb variants.
MilliporeSigma™ Supelco™ Ascentis™ Express HILIC Guard Cartridge, 2 μm Particle Size
Ascentis™ Express Guard Columns provide physical (filtration) and chemical protection for costly analytical columns without compromising the very high performance of Ascentis™ Express columns.
MilliporeSigma™ Supelco™ apHera™ NH2 HPLC Guard Column, 5 μm Particle Size
apHera™ amino columns are based on covalently bonded polyamine specifically optimized for the separation of mono- and oligosaccharides.
MilliporeSigma™ Supelco™ Ascentis™ Express OH5 Guard Cartridge, 2 μm Particle Size
Ascentis™ Express Guard Columns provide physical (filtration) and chemical protection for costly analytical columns without compromising the very high performance of Ascentis™ Express columns.
MilliporeSigma™ Supelco™ SRT™-C SEC-300 Gel Filtration Guard Column, 5 μm Particle Size, Stainless Steel
The bonded phase in SRT™-C SEC columns is more polar than the hydrophilic phase used in SRT™ columns, making SRT™-C columns better suited for the gel filtration analysis of more hydrophobic bioopolymers such as membrane proteins and pegylated antibodies.
MilliporeSigma™ Supelco™ SRT™-C SEC-500 Gel Filtration Guard Column, 5 μm Particle Size, Stainless Steel
The bonded phase in SRT™-C SEC columns is more polar than the hydrophilic phase used in SRT™ columns, making SRT™-C columns better suited for the gel filtration analysis of more hydrophobic bioopolymers such as membrane proteins and pegylated antibodies.
MilliporeSigma™ Supelco™ BIOshell™ A400 Protein C18 Guard Column, 3.4 μm Particle Size
BIOshell™ Guard Columns provide physical (filtration) and chemical protection for costly analytical columns without compromising the very high performance of BIOshell™ columns.
MilliporeSigma™ Supelco™ Proteomix™ SCX-NP1.7 Strong Cation Exchange Guard Cartridge and Holder, 1.7 μm Particle Size, Stainless Steel
Used for the analyis or small scale purification of negatively charged biopolymers, such as glycoprotein isoforms, pegylated proteins and oligonucleotide.
MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-8-DB Supelguard Cartridge Kit, 5 μm Particle Size
SUPELCOSIL™ LC-DB phases are specially deactivated for basic compounds. These columns provide less retention, better peak shape, and higher efficiency for organic bases than can be obtained on conventional reversed-phase columns.
MilliporeSigma™ Supelco™ Discovery™ HS F5 Supelguard™ Cartridge Kit, 3 μm Particle Size
The Discovery™ HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.