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HPLC Columns and Cartridges

Pre-packed columns, cartridges, and guard columns for separation of biomolecules and organic/inorganic analytes; includes normal/reversed phase HPLC, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
HPLC Columns (3,956)
HPLC Guard Columns and Cartridges (551)
HPLC Guard Column Accessories (33)

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1 – 15 of 1,268 results
1

Thermo Scientific™ BetaBasic™ Phenyl Columns, 5μm Particle Size

BetaBasic columns are highly efficient and reproducible for small molecules, peptides and protein digests

Pore Size 150 Å
Particle Size 5 μm
Carbon Load 7%
Stationary Phase Phenyl (Ph)
Phase Reversed Phase
For Use With LC/MS Separation
Endcapped Yes
Packing Material Silica
Column Type Reversed Phase
Surface Area 200 m²/g
Particle Shape Spherical
Film Thickness 5 μm
USP Type L11
2

Thermo Scientific™ BetaBasic™ 8 Columns, 3μm Particle Size

Available in limited dimensions. Please contact Customer Service for more details.

Pore Size 150 Å
Particle Size 3 μm
Carbon Load 7%
Stationary Phase C8
Phase Reversed Phase
For Use With LC/MS, APCI
Endcapped Yes
Packing Material Silica
Column Type Reversed Phase
pH 2 to 9
Surface Area 200 m²/g
USP Type L7
3

MilliporeSigma™ Supelco™ SRT™-C SEC-1000 Gel Filtration Guard Column, 5 μm Particle Size, PEEK

The bonded phase in SRT™-C SEC columns is more polar than the hydrophilic phase used in SRT™ columns, making SRT™-C columns better suited for the gel filtration analysis of more hydrophobic bioopolymers such as membrane proteins and pegylated antibodies.

4

MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C18 Supelguard™ Cartridge Kit, 3 μm Particle Size

Kits include one cartridge, a stand-alone holder, a piece of tubing, and 2 nuts and ferrules. Guard cartridges require holders that are sold separately.

Includes 1 cartridge, a stand-alone holder, a piece of tubing, and 2 nuts and ferrules
Pore Size 300 Å
Particle Size 3 μm
Type Guard Cartridge Kit
Phase Reversed Phase
Length (Metric) 2 cm
Quantity 1 Kit
For Use With (Application) HPLC
Product Line Discovery Supelguard
5

MilliporeSigma™ Supelco™ Ascentis™ C18 Supelguard™ Guard Cartridge Kit, 3 μm Particle Size

Ascentis™ C18 is an extremely stable and reliable HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.

Includes 1 cartridge, a stand-alone holder, a piece of tubing, and 2 nuts and ferrules (10 mm i.d guard requires the purchase of 567499-U)
Pore Size 100 Å
Particle Size 3 μm
Type Fused-Core HPLC Guard Cartridge Kit
Phase Reversed Phase
Length (Metric) 2 cm
Endcapped Yes
For Use With (Equipment) Ascentis C18 Columns
pH 2 to 8
Quantity 1 Kit
For Use With (Application) HPLC
Surface Area 450 m2/g
Product Line Ascentis Supelguard
6

MilliporeSigma™ Supelco™ ChromegaChiral™ Chiral CCO F4 HPLC Column, 3 μm Particle Size

ChromegaChiral™ CCO F4 is a 4-Fluoro 3-methylphenyl cellulose phase which can be used in SFC or HPLC.

Pore Size 1000 Å
Particle Size 3 μm
Stationary Phase 2-fluoro 5-methylphenyl cellulose
Phase Chiral
Matrix 2-fluoro 5-methylphenyl cellulose Phase
Technique Chiral HPLC
Product Line ChromegaChiral
7

MilliporeSigma™ Supelco™ CHIRALPAK™ HSA HPLC Column, 5 μm Particle Size

CHIRALPAK™ HSA, which uses human serum albumin as the chiral selector, is highly selective for acidic racemates, preferably weak and strong acids, zwitterionic and non-protolytic (neutral) compounds.

Particle Size 5 μm
Stationary Phase Human Serum Albumin
Phase Chiral
Matrix Human Serum Albumin Phase
Technique Chiral HPLC
Product Line CHIRALPAK
8

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-18-S Supelguard Cartridge, 5 μm Particle Size

SUPELCOSIL™ LC-18-S Supelguard™ Cartridges contain a SUPELCOSIL™ packing, in a 2 cm stainless steel body, enclosed by polyether ether ketone (PEEK) encapsulated stainless steel frits.

Pore Size 120 Å
Particle Size 5 μm
Type Guard Cartridge
Phase Reversed Phase
Length (Metric) 2 cm
Quantity 2/Pk.
For Use With (Application) HPLC
Product Line SUPELCOSIL Supelguard
9

MilliporeSigma™ Supelco™ SUPELCOSIL™ Suplex™ pKb-100 Supelguard Cartridge Kit, 5 μm Particle Size

Suplex™ pKb-100 columns feature the same bonded phase functionality as SUPELCOSIL™ LC-ABZ columns.

10

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-NH2 Supelguard Cartridge Kit, 5 μm Particle Size

SUPELCOSIL™ LC-NH2 Supelguard™ Cartridges contain a SUPELCOSIL™ packing, in a 2 cm stainless steel body, enclosed by polyether ether ketone (PEEK) encapsulated stainless steel frits (2 μm porosity).

11

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-NH2-NP Supelguard Cartridge, 5 μm Particle Size

SUPELCOSIL™ LC-NH2 Supelguard™ Cartridges contain a SUPELCOSIL™ packing, in a 2 cm stainless steel body, enclosed by polyether ether ketone (PEEK) encapsulated stainless steel frits (2 μm porosity).

Particle Size 5 μm
Type Guard Cartridge
Phase Reversed Phase
Length (Metric) 2 cm
Diameter (Metric) Inner 4 mm
For Use With (Application) HPLC
Product Line SUPELCOSIL Supelguard
12

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-Si Supelguard Cartridge, 5 μm Particle Size

A typical mobile phase used with normal-phase silica columns consists of a hydrocarbon solvent such as hexane or heptane, mixed with a relatively small percentage of more polar solvent.

14

MilliporeSigma™ Supelco™ Ascentis™ Express F5 HPLC Column, 2.7 μm Particle Size

Ascentis™ Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

15

MilliporeSigma™ Supelco™ Proteomix™ WCX-NP3 Weak Cation Exchange Column, 3 μm Particle Size, PEEK

Used for the analysis or small scale purification of positively charged biopolymers including glycoprotein isoforms and mAb variants.

Pore Size Nonporous
Particle Size 3 μm
Stationary Phase Weak Cation Exchange
Phase Cation Exchange
Matrix Spherical PS-DVB/Weak Cation Exchange Phase
Length (Metric) 5 cm
pH 2.0 to 12.0
Material PEEK
Technique Cation Exchange
Product Line Proteomix