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HPLC Columns and Cartridges

Pre-packed columns, cartridges, and guard columns for separation of biomolecules and organic/inorganic analytes; includes normal/reversed phase HPLC, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
HPLC Columns (3,957)
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1 – 15 of 1,263 results
1

Thermo Scientific™ GuardGOLD™ Capillary Columns

Protect your analytical column from damage and contamination from nonvolatile materials.

2

MilliporeSigma™ Supelco™ Kromasil C18 HPLC Column, 100 Å, 3.5 μm Particle Size

For small molecules and peptides.

Pore Size 100 Å
Particle Size 3.5 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase
Product Line Kromasil
3

MilliporeSigma™ Supelco™ General-Purpose Chromatography columns

For use in general chromatographic work for extraction of components, with special applications in isoenzyme and protein studies, hemoglobin fractionation and desalting.

Column Type Chromatography
Material Polypropylene
4

MilliporeSigma™ Supelco™ Discovery™ HS F5 HPLC Column, 5 μm Particle Size

The Discovery™ HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.

Pore Size 120 Å
Particle Size 5 μm
Stationary Phase PFP (Pentafluorophenyl)
Phase Reversed Phase and HILIC
Matrix PFP (Pentafluorophenyl) Phase/Silica Gel, High Purity, Spherical Particle Platform
Endcapped Yes
pH 2 to 8
Surface Area 300 m2/g
Product Line Discovery
5

MilliporeSigma™ Supelco™ Antibodix™ WCX-NP10 Weak Cation Exchange Column, 10 μm Particle Size, PEEK

Antibodix™ columns are designed for the reproducible analysis of charge variants of monoclonal antibodies by salt or pH gradient.

Pore Size Nonporous
Particle Size 10 μm
Stationary Phase Spherical PS-DVB
Phase Cation Exchange
Matrix Spherical PS-DVB Phase
pH 2.0 to 12.0
Material PEEK
Technique Cation Exchange
Product Line Antibodix
7

Thermo Scientific™ Single nanoViper™ and Double nanoViper™ PepMap™ Neo UHPLC Columns

Separate challenging peptide mapping samples with Thermo Scientific™ Double nanoViper™ PepMap™ Neo UHPLC columns.

Pore Size 100 Å
Particle Size 2 μm
Carbon Load 15%
Stationary Phase C18
Temperature 60°C
Endcapped Yes
Packing Material Spherical, Fully Porous, Ultrapure Silica
Column Type Reversed Phase
pH 2 to 8
For Use With (Application) Peptide Mapping, LC-MS
Max. Pressure 22,000 psi (1500 bar)
USP Type L1
8

MilliporeSigma™ Supelco™ SRT™ SEC-2000 Gel Filtration Column, 5 μm Particle Size, Stainless Steel

Sepax SRT™ SEC columns feature a hydrophilic surface layer that effectively shields the highly porous, five micron silica particles from interacting with your sample.

Pore Size 2000 Å
Particle Size 5 μm
Stationary Phase Hydrophilic Polymer
Phase Size Exclusion
Matrix Hydrophilic Polymer Phase/Spherical Silica
Length (Metric) 30 cm
pH 2.0 to 8.5
Material Stainless Steel
Technique SEC HPLC
Product Line SRT
9

Thermo Scientific™ Acclaim™ HILIC-10 HPLC Columns
GREENER_CHOICE

Use this HILIC column to retain and separate highly polar molecules that are unretained by reversed-phase chromatography.

Pore Size 120 Å
Carbon Load 8%
Stationary Phase Proprietary
Temperature 70°C
Phase HILIC
For Use With LC/MS
Endcapped Yes
Column Type HILIC
pH 2 to 8
Surface Area 300 m²/g
Product Line Acclaim
10

Thermo Scientific™ ProPac™ Elite WCX HPLC Column
GREENER_CHOICE

This column provides consistent separations of therapeutic proteins including monoclonal antibodies, antibody drug conjugates, biosimilars and charge variants.

Pore Size Nonporous
Stationary Phase Carboxylate
Temperature 10°C to 60°C
Phase Ion Exchange
Column Type Ion Exchange
Green Features FSC
Max. Pressure 4500 psi (310 bar)
Column Format Analytical Column
Particle Size 5 μm
Packing Material Nonporous DVB Particles with Hydrophilic Coating
Mobile Phase Compatibility Recommended: Goods Buffers and NaCl. Minimum 20 mM salt required. Do not rinse with pure DI H2O
pH 2 to 12
Technique Bio-LC
Product Line ProPac
11

TSKgel™ Super-Phenyl HPLC Column, 2.3 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ Super-Phenyl Columns are packed with monodispersed 2 μm (nominal, mean particle size is 2.3 μm) spherical silica particles covalently bonded with phenyl groups.

Shipping Condition Shipped in 50% Acetonitrile, 50% Water
Particle Size 2.3 μm
Solvents 0-100% Organic Solvent
Carbon Load 3%
Stationary Phase Phenyl
Temperature 10°C to 50°C
Phase Reversed Phase
Matrix Phenyl Phase/Silica Particle Platform
Endcapped Yes
pH 2 to 7.5
Surface Area 100 m2/g
Product Line TSKgel
12

MilliporeSigma™ Supelco™ Ascentis™ C8 HPLC Column, 5 μm Particle Size

The Ascentis™ C8 is a highly reproducible column with excellent selectivity towards polar compounds. The phase excels in highly aqueous mobile phases as well as high organic mobile phases.

Pore Size 100 Å
Particle Size 5 μm
Stationary Phase C8 (Octyl)
Phase Reversed Phase
Matrix C8 (Octyl) Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 8
Technique Fused-Core HPLC
Surface Area 450 m2/g
Product Line Ascentis
13

MilliporeSigma™ Supelco™ LiChrospher™ RP-8 HPLC Column, 5 μm Particle Size

LiChrospher™ RP-8 HPLC Column is a traditionally produced spherical silica gel carrier with C-8 reversed-phase properties and is suitable for acidic, neutral and weakly basic compounds.

Pore Size 100 Å
Particle Size 5 μm
Stationary Phase C8 (Octyl)
Phase Reversed Phase
Matrix C8 (Octyl) Phase
Endcapped No
Surface Area 350 m2/g
Product Line LiChrospher
14

TSKgel™ DEAE-SW Columns, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ DEAE-2SW columns are packed with 5 μm porous spherical silica beads which are surface modified with a weak anion exchanger. They are used for the separation and analysis of nucleotides, nucleosides, peptides, small proteins and other biologically active molecules. A 2 mm ID column is available for applications that are sample limited.

15

Thermo Scientific™ BetaSil™ C8 HPLC Columns, 5μm Particle Size

High surface area with high bonded phase coverage

Pore Size 100 Å
Stationary Phase C8
Phase Reversed Phase
For Use With LC/MS Separation
Endcapped Yes
Column Type Reversed Phase
Column Chemistry Octyl
Surface Area 310 m²/g
Particle Size 5 μm
Carbon Load 12%
Packing Material Silica
pH 2 to 9
Particle Shape Spherical
Film Thickness 5 μm
USP Type L7