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HPLC Columns and Cartridges

Pre-packed columns, cartridges, and guard columns for separation of biomolecules and organic/inorganic analytes; includes normal/reversed phase HPLC, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
HPLC Columns (3,969)
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HPLC Guard Column Accessories (33)

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1 – 15 of 1,266 results
1

Thermo Scientific™ Hypersil™ ODS C18 Columns

Use as a general purpose C18 phase for acidic and neutral analytes.

Pore Size 120 Å
Carbon Load 10%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 170 m²/g
USP Type L1
2

Thermo Scientific™ Hypersil GOLD™ aQ Javelin™ Guard HPLC Columns

Javelin guard columns offer convenient, easy to use protection for analytical HPLC columns

3

Thermo Scientific™ GuardGOLD™ Capillary Columns

Protect your analytical column from damage and contamination from nonvolatile materials.

4

MilliporeSigma™ Supelco™ Astec™ Cellulose DMP Chiral HPLC Column, 5 μm Particle Size

Description: Spherical, porous silica coated with DMPC (dimethylphenyl carbamate)-derivatized cellulose. Field of Use: Small molecule enantiomer separations in normal phase, polar organic, and SFC modes.

Particle Size 5 μm
Stationary Phase Cellulose, DMPC-
Phase Chiral
Matrix Cellulose, DMPC- Phase/Silica Gel High Purity, Spherical/Silica Gel Particle Platform
Technique Chiral HPLC
Product Line Astec CYCLOBOND
5

MilliporeSigma™ Supelco™ Proteomix™ HIC Butyl-NP5 Column, 5 μm Particle Size

Proteomix™ HIC columns are specially designed for high resolution and high efficiency separations of proteins, oligonucleotides and peptides.

Pore Size Nonporous
Particle Size 5 μm
Stationary Phase C4 (butyl)
Phase Hydrophobic Interaction
Matrix C4 (Butyl) Phase/Polystyrene/Divinylbenzene Particle Platform (Spherical, highly cross-linked)
Material Stainless Steel Hardware
Technique HIC HPLC
Product Line Proteomix
6

MilliporeSigma™ Supelco™ Ascentis™ Express C8 Capillary HPLC Column, 2.7 μm Particle Size

Ascentis™ Express HPLC columns, through the use of Fused-Core™ particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures.

Pore Size 90 Å
Particle Size 2.7 μm
Stationary Phase C8 (Octyl)
Phase Reversed Phase
Matrix C8 (Octyl) Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
7

MilliporeSigma™ Supelco™ BIOshell™ A400 Protein C18 Column, 3.4 μm Particle Size (Dia. 75-300 μm)

BIOshell™ 400 Å Protein C18 columns contain 3.4 μm advanced Fused-Core™ particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins.

Particle Size 3.4 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/Spherical Silica Particle Platform
Endcapped Yes
pH 1 to 9
Material Stainless Steel
Technique Fused-Core HPLC
Surface Area ̃15 m2/g
Product Line BIOshell
8

MilliporeSigma™ Supelco™ TSKgel™ ODS-100Z HPLC Column phase C18 (octadecyl), 3 μm Particle Size, Stainless Steel

This general purpose reversed phase column is the workhorse for analysis of small molecular weight compounds in life science applications.

9

MilliporeSigma™ Supelco™ Partisil™ 10 SAX HPLC Column, 85 Å, 10 μm Particle Size

Partisil™ 10 SAX HPLC column is a strong anion exchanger useful for the separation of nucleotides, nucleosides and bases.

10

MilliporeSigma™ Supelco™ Ascentis™ C8 HPLC Column, 3 μm Particle Size

The Ascentis™ C8 is a highly reproducible column with excellent selectivity towards polar compounds. The phase excels in highly aqueous mobile phases as well as high organic mobile phases.

Pore Size 100 Å
Particle Size 3 μm
Stationary Phase C8 (Octyl)
Phase Reversed Phase
Matrix C8 (Octyl) Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 8
Technique Fused-Core HPLC
Surface Area 450 m2/g
Product Line Ascentis
11

MilliporeSigma™ Supelco™ Proteomix™ HIC Ethyl-NP5 Column, 5 μm Particle Size

Proteomix™ HIC columns are specially designed for high resolution and high efficiency separations of proteins, oligonucleotides and peptides.

Pore Size Nonporous
Particle Size 5 μm
Stationary Phase Ethyl
Phase Hydrophobic Interaction
Matrix Ethyl Phase/Polystyrene/Divinylbenzene Particle Platform (Spherical, highly-crossed linked)
Material Stainless Steel Hardware
Technique HIC HPLC
Product Line Proteomix
12

MilliporeSigma™ Supelco™ SUPELCOGEL™ 8Ca, 8% Crosslinked HPLC Column, 9 μm Particle Size

Offers excellent separation of many common samples.

Particle Size 9 μm
Stationary Phase Sulfonated Polystyrene
Phase Ion Exclusion
Matrix Sulfonated Polystyrene/Divinylbenzene Phase/Spherical Particle Platform
Crosslinking 0.08
Technique Crosslinked HPLC
Product Line SUPELCOGEL
13

MilliporeSigma™ Supelco™ Proteomix™ SCX-NP3 Strong Cation Exchange Column, 3 μm Particle Size, Stainless Steel

Used for the analysis or small scale purification of positively charged biopolymers including glycoprotein isoforms and mAb variants.

Pore Size Nonporous
Particle Size 3 μm
Stationary Phase Sulfonic Acid
Phase Cation Exchange
Matrix Spherical PS-DVB/Sulfonic Acid Phase
pH 2.0 to 12.0
Material Stainless Steel
Technique Cation Exchange
Product Line Proteomix
15

MilliporeSigma™ Supelco™ Ascentis™ Express HILIC UHPLC Column, 2 μm Particle Size

Contains a bare silica stationary phase that can be used for traditional normal phase separations or aqueous normal phase chromatography.

Pore Size 90 Å
Particle Size 2 μm
Stationary Phase Silica
Phase Reversed Phase
Matrix Silica Phase/Fused-Core Particle Platform
Endcapped No
Technique Fused-Core UHPLC
Product Line Ascentis