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HPLC Columns and Cartridges

Pre-packed columns, cartridges, and guard columns for separation of biomolecules and organic/inorganic analytes; includes normal/reversed phase HPLC, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
HPLC Columns (3,946)
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1 – 15 of 1,261 results
1

Thermo Scientific™ Acclaim™ C30 Reversed Phase HPLC Column, 3 μm, 3 mm x 100 mm

Column Format: Analytical Column; Carbon Load: 13%; Pore Size: 200 Å; Column Phase: Reversed Phase

2

MilliporeSigma™ Supelco™ Hamilton™ PRP-X100 HPLC Column, 10 μm Particle Size

The Hamilton PRP-X100, polymeric HPLC columns used for the separation of anions in ion chromatography.

3

MilliporeSigma™ Supelco™ SUPELCOSIL™ LC-NH2 HPLC Column, 5 μm Particle Size

The amino column is most often employed for the separation of mono- and disaccharides.

Pore Size 120 Å
Particle Size 5 μm
Stationary Phase Amino (NH2)
Phase Reversed Phase
Matrix Amino (NH2) Phase/Silica Gel, Spherical Particle Platform
Length (Metric) 25 cm
Endcapped Yes
Surface Area 170 m2/g
Product Line SUPELCOSIL
4

MilliporeSigma™ Supelco™ Ascentis™ Express C8 HPLC Column, 2.7 μm Particle Size

Ascentis™ Express HPLC columns, through the use of Fused-Core™ particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures.

Pore Size 90 Å
Particle Size 2.7 μm
Stationary Phase C8 (Octyl)
Phase Reversed Phase
Matrix C8 (Octyl) Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
5

MilliporeSigma Ascentis™ Express PCS-C18 HPLC Column

Ascentis™ Express PCS-C18 columns are specifically engineered to provide efficient separation of basic, acidic, or neutral compounds under low ionic strength (formic acid) mobile phase conditions.

Pore Size 90 Å
Particle Size 2.7 μm
Type HPLC Column
Phase Positively Charged Surface (PCS)
Material Stainless Steel
Technique HPLC
6

MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C5 HPLC Column, 10 μm Particle Size

Discovery™ BIO Wide Pore C5 was designed for the efficient and reliable separation of proteins and peptides, especially hydrophobic peptides, by RP- HPLC.

Pore Size 300 Å
Particle Size 10 μm
Stationary Phase C5 (pentyl)
Phase Reversed Phase
Matrix C5 (pentyl) Phase/Silica Particle Platform
Endcapped Yes
Technique Wide Pore HPLC
Surface Area 100 m2/g
Product Line Discovery
7

MilliporeSigma™ Supelco™ ChromegaChiral™ Chiral CCA HPLC Column, 3 μm Particle Size

A polysaccharide coated chiral stationary phase and columns which are produced using a unique production process of coating the proven chiral selector, tris-(3,5-di- methylphenyl) carbamoyl amylose on high purity silica gel.

Pore Size 1000 Å
Particle Size 3 μm
Stationary Phase carbamoyl
Phase Chiral
Matrix carbamoyl Phase
Technique Chiral HPLC
Product Line ChromegaChiral
8

MilliporeSigma™ Supelco™ Ascentis™ Si HPLC Column, 3 μm Particle Size

The Ascentis™ Si is a high loading capacity silica with excellent peak shape. The Ascentis™ Si performs in both normal-phase and HILIC/ANP (aqueous normal phase) mode.

Pore Size 100 Å
Particle Size 3 μm
Stationary Phase SIlica
Phase Reversed Phase
Matrix Silica Gel High Purity, Spherical/Silica Phase
Endcapped No
pH 2 to 6
Technique Fused-Core HPLC
Surface Area 450 m2/g
Product Line Ascentis
9

Thermo Scientific™ Hypersil GOLD™ PEI HILIC HPLC Columns

Retain highly polar and hydrophilic analytes, which are difficult to analyze with conventional C18 columns, using Hypersil GOLD PEI HILIC LC Columns.

Pore Size 175 Å
Carbon Load 6%
Stationary Phase PEI HILIC
Temperature 60°C
Phase HILIC
Endcapped No
pH 2 to 8
Surface Area 220 m²/g
10

TSKgel™ SuperH3000 HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ SuperH3000 Columns are packed with rigid, porous, polymer beads. They are used for the non-aqueous separation of polymers with molecular weights less than 60,000 Da.

11

MilliporeSigma™ Supelco™ SUPELCOGEL™ H, 6% Crosslinked HPLC Column, 9 μm Particle Size

Used for the analysis of saccharides or organic acids.

Particle Size 9 μm
Stationary Phase Sulfonated Polystyrene
Phase Ion Exclusion
Matrix Sulfonated Polystyrene/Divinylbenzene Phase/Spherical Particle Platform
Crosslinking 0.06
Technique Crosslinked HPLC
Product Line SUPELCOGEL
12

Thermo Scientific™ Hypercarb™ HPLC Column, 5μm

Thermo Scientific™ Hypercarb™ Columns give you 100% porous graphitic carbon for extended separation capabilities

Pore Size 250 Å
Particle Size 5 μm
Carbon Load 100%
Phase Normal and Reversed Phase
Column Type Mixed Mode
pH 1 to 14
Surface Area 120 m²/g
USP Type L109
Column Format Analytical Column
13

MilliporeSigma™ Supelco™ BIOshell™ A400 Protein C18 Column, 3.4 μm Particle Size (Dia. 1-4.6 mm)

BIOshell™ 400 Å Protein C18 columns contain 3.4 μm advanced Fused-Core™ particles with 400 Å pores that are specifically engineered to provide efficient separation of larger proteins.

Particle Size 3.4 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/Spherical Silica Particle Platform
Endcapped Yes
pH 1 to 9
Material Stainless Steel
Technique Fused-Core HPLC
Surface Area ̃15 m2/g
Product Line BIOshell
14

MilliporeSigma™ Supelco™ BIOshell™ A160 Peptide C18 HPLC Column, 160 Å, 2.7 μm Particle Size

BIOshell™ 160 Å Peptide C18 columns are specifically engineered to provide efficient separation of peptides as well as small proteins.

Pore Size 160 Å
Particle Size 2.7 μm
Carbon Load 4.6%
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/Fused-Core Particle Platform
Endcapped No
pH 1 to 8
Material Stainless Steel Hardware
Technique Fused-Core HPLC
Surface Area 90 m2/g
Product Line BIOshell
15

Thermo Scientific™ BetaBasic™ Javelin™ C18 Reversed Phase HPLC Column, 5 μm, 4 mm x 20 mm

Column Format: Guard Column; Carbon Load: 13%; Pore Size: 150 Å; Column Phase: Reversed Phase