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Additional Cell Culture Reagents and Supplements

A broad array of reagents that support specific cell culture operations, processes, and analytics. Products included may promote adherence and spreading, nurture specific cell lines, or support transfection processes.
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1
Promotions Available

Cytiva HyClone™ HEPES Solution

Maintains physiological pH in cell culture, despite changes in carbon dioxide concentration (produced by cellular respiration). HyClone™ HEPES Solution is part of high-performance line of nutritional and growth supplements.

EDGE
2
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Cytiva HyClone™ Dulbecco's Phosphate Buffered Saline, Solution

Buffer cell culture with PBS in solution.

EDGE
Regulatory Status RUO
Content And Storage 15° to 30°C
Sterilization Method 0.1 μm Sterile-filtered
Product Type Dulbecco's Phosphate Buffered Saline
Form Liquid
Endotoxin Level ≤0.025EU/mL
pH 7 to 7.6
Shelf Life 24 Months
Certifications/Compliance Manufactured under cGMP and ISO 9001 certified facilities
For Use With (Application) Serves as an irrigating, transporting or diluting fluid while maintaining cell tonicity and viability for a limited period of time
Osmolality 276±10mOsm/kg H2O
Sterility Sterile
3

MilliporeSigma™ Poly-L-Lysine Solution

Used as coating to enhance cell attachment and adhesion to both plasticware and glass surfaces

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4

MilliporeSigma™ Polybrene Infection/Transfection Reagent

A highly efficient method of gene transfer into mammalian cells leveraging infection with retroviral vectors.

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5
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Cytiva HyClone™ 100mM Sodium Pyruvate Solution

Filter sterilized. HyClone™ 100mM Sodium Pyruvate solution is an easily accessible carbohydrate source that may also have protective effects against hydrogen peroxide.

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6
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Cytiva HyClone™ Phosphate Buffered Saline (PBS)

Buffer cell culture growth with PBS

EDGE
Content And Storage 15° to 30°C
Sterilization Method 0.1 μm Sterile-filtered
Product Type Phosphate Buffered Saline (PBS)
Form Liquid
Without Additives No Calcium,No Magnesium,No Phenol Red
Endotoxin Level =1.0EU/mL
pH 7.0 to 7.2
Certifications/Compliance cGMP and ISO 9001:2000 Guidelines
7

Gibco™ GlutaMAX™ Supplement  DFS Item
GREENER_CHOICE

GlutaMAX Supplement is an L-alanyl-L-glutamine dipeptide alternative to L-glutamine. It exhibits increased stability over L-glutamine leading to improved cellular health.

Content And Storage Storage conditions: 15°C to 30°C
Alternate storage conditions: -5°C to -20°C and 2°C to 8°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
Product Type GlutaMax
Form Liquid
Classification Animal Origin-free
Manufacturing Quality cGMP-compliant under the ISO 13485 standard
Culture Type Mammalian Cell Culture
Shelf Life 24 Months
For Use With (Application) Clinical Research, Translational Research
Sterility Sterile-filtered
Product Line Gibco, GlutaMAX
Cell Type Mammalian Cells
8

Sartorius NexaGel Cell Recovery Solution

Non-enzymatic, ready-to-use cell recovery solution designed for the rapid and efficient harvesting of organoids or 3D cells cultured with NexaGel™or animal-based ECM.

Form Liquid
9
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Cytiva HyClone™ Cell Boost™ 7b Supplement

A concentrated amino acid supplement, in a ready to use powder format, for high-yield protein production in CHO fed-batch processes

Description Without: L-glutamine, poloxamer 188, and insulin
Product Type Amino Acid Supplement
For Use With (Application) High-Yield Protein Production in CHO Fed-Batch Processes
Product Line HyClone
10

Gibco™ CTS™ HBSS

Gibco Cell Therapy Systems (CTS) Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.

Shipping Condition Ambient
Content And Storage 15°C to 30°C
Sterilization Method 0.2 μm Sterile-filtered
Form Clear, colorless solution
Sterility Sterile-filtered
13

Gibco™ EfficientFeed™ B+ 3X Supplement

Gibco EfficientFeed B+ 3X Supplement is a single-part liquid supplement for fed batch addition to the culture of multiple cell lines to assist with process development and maximization of bioreactor utilization.

Shipping Condition Approved for shipment at Room Temperature or on Wet Ice
Content And Storage Store at 2–8°C. Store dark and dry.
Product Type Cell Culture Supplement
Form Liquid
pH 6 to 8
Shelf Life 12 Months
Product Line EfficientFeed
15

MilliporeSigma™ Ndiff™ Chemicon™ Neuro-27 Medium Supplement (100x)

a serum-free supplement containing antioxidants and other factors that has been developed for the in vitro propagation and maintenance of undifferentiated murine embryonic stem (ES) cells in serum free medium.