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Gibco™ Poly-D-Lysine

Catalog No. A3890401
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100 mL
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A3890401 100 mL
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Catalog No. A3890401 Supplier Gibco™ Supplier No. A3890401
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Description

Poly-D-Lysine is an extracellular matrix used to facilitate neural cell adhesion to tissue culture-treated plastic and glass surfaces. When used on Nunc plastics with B-27 supplements or the B-27 Plus Neuronal Culture System, Poly-D-Lysine maintains cell viability and normal growth characteristics in multiple neuronal cell types.

Poly-D-Lysine is a chemically synthesized extracellular matrix used to facilitate cell adhesion to tissue culture treated plastic and glass surfaces. Poly-D-Lysine contains polymers with a molecular weight between 50,000 – 150,000 Daltons which makes this product ideal for neuronal culture applications. This ready-to-use product is provided as a liquid at a concentration of 0.1 mg/mL. When used on Nunc cell culture plastics with B-27 Supplements or the B-27 Plus Neuronal Culture System, Poly-D-Lysine has been proven to maintain cell viability and normal growth characteristics in multiple neuronal cell types. Key features include:

  • Synthetic and animal origin-free
  • Sterile-filtered for cell culture applications
  • Validated with B-27 Supplement and B-27 Plus Neuronal Culture System when used with Nunc plastic and glass bottom dishes

Specifications

Content And Storage Store in refrigerator (2–8°C).
Cell Type Mammalian Cells
Classification Animal Origin-free
Concentration 0.1 mg/mL
Culture Type Mammalian Cell Culture
Form Liquid
Product Type Extracellular Matrix
Sterility Sterile
Product Line Gibco
Purity or Quality Grade Cell Culture Grade
Quantity 100 mL
Shipping Condition Approved for shipment on Wet Ice
Source Animal origin-free, Synthetic
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Frequently Asked Questions (FAQs)

What solution is Poly-D-Lysine (Cat. No. A3890401) delivered in?

Poly-D-Lysine is in PBS solution.
Can I freeze Poly-D-Lysine?

Yes, Poly-D-Lysine can undergo one freeze-thaw cycle without impacting performance. Please note that this does not extend the shelf-life of the product.

Are there any animal origin components in Poly-D-Lysine?

The Poly-D-Lysine matrix is animal origin-free.

I coated my plates with Poly-D-Lysine and I'm noticing that the cells are dying within a few days of plating the cells. How can I ensure cell survival?

It is possible that the plates were not adequately rinsed with water prior to plating the cells. Residual Poly-D-Lysine is toxic and may be the cause of cell death. It is recommended to rinse the plates with water three times before exposing the cells to the matrix.
I coated my plates with Poly-D-Lysine and I'm noticing that the cells are not attaching well. What can I do to prevent the cells from lifting off?

When coating the surface with Poly-D-Lysine, make sure that the entire surface is fully covered and that the appropriate volume of coating matrix is applied.

What type of cultureware can I pair with Poly-D-Lysine?

The Poly-D-Lysine matrix has been coated on a variety of Nunc cell cultured treated plastic multi-well dishes as well as glass surfaces.
Can I use the Poly-D-Lysine matrix with other coatings?

iPSC-derived neurons have been cultured successfully on Poly-D-Lysine with a secondary coating of mouse and human laminin.
I'm currently culturing neurons. Which media system do you recommend using in conjunction with the Poly-D-Lysine matrix?

We recommend using the B-27 Plus Neuronal Culture System when culturing neurons on Poly-D-Lysine. The B-27 Plus Neuronal Culture System is a complete media system that includes the B-27 Plus Supplement and the Neurobasal Plus Medium.
What cell types will Poly-D-Lysine support as an attachment matrix?

The Poly-D-Lysine matrix has successfully supported the attachment of primary rodent neurons, iPSC-derived neurons and PC12 cells.
How long can I store Poly-D-Lysine at 4 degrees C?

The product can be stored at 4 degrees C for 12 months after the date of production.

For Research Use Only. Not for use in diagnostic procedures.