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Gibco™ GlutaMAX™ Supplement
Description
Gibco GlutaMAX Supplement is an L-alanyl-L-glutamine dipeptide alternative to L-glutamine that exhibits increased stability, leading to improved cell health. L-Glutamine is an amino acid required in cell culture for the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, and other amino acids, as well as in protein synthesis and glucose production. GlutaMAX Supplement is suitable for adherent and suspension culture of mammalian cells, with no adaptation required. It is formulated as a 200 mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl.
Compared to L-glutamine, GlutaMAX Supplement:
- Minimizes toxic ammonia build-up
- Improves cell viability and growth
- Remains stable across a wide range of temperatures
Minimizes toxic ammonia build-up
Unlike L-glutamine, GlutaMAX Supplement does not spontaneously break down to form ammonia. Instead, cells cleave the dipeptide bond to release L-glutamine as needed. This system prevents waste build-up and maintains a fresh supply of L-glutamine during long-term culture.
Improves cell viability and growth
Cultures with lower levels of ammonia show improved cell viability. In addition, culture conditions are more controlled due to the consistent concentration of GlutaMAX Supplement that is not possible with L-glutamine.
Remains stable across a wide range of temperatures
GlutaMAX Supplement is room-temperature stable and ready to use. It retains full functionality for 24 months at room temperature, 2 to 8°C, or -5 to -30°C for convenient storage and handling. GlutaMAX Supplement is even stable at 37°C for a week or more.
Order Info
Shipping Condition: Room Temperature
Specifications
Specifications
| Content And Storage | Storage conditions: 15°C to 30°C Alternate storage conditions: -5°C to -20°C and 2°C to 8°C Shipping conditions: Room temperature Shelf life: 24 months from date of manufacture |
| Cell Type | Mammalian Cells |
| Classification | Animal Origin-free |
| Concentration | 100X |
| Culture Type | Mammalian Cell Culture |
| Form | Liquid |
| Product Type | GlutaMax |
| Sterility | Sterile-filtered |
| For Use With (Application) | Clinical Research, Translational Research |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
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Frequently Asked Questions (FAQs)
GlutaMAX Supplement (Cat. No. 35050061, 35050079) is highly recommended to reconstitute Geltrex Reduced Growth Factor Basement Membrane Matrix because it offers higher stability than L-glutamine. The L-glutamine in Geltrex matrix comes in the form of the dipeptide L-alanyl-L-glutamine, which degrades slower than L-glutamine in cell culture. While GlutaMAX supplement is highly recommended, it is not required for Geltrex matrix–based culture systems.
When using Dynamis AGT Medium (Cat. Nos. A2617501, A2617502, A2617503, A2617504) for culturing a CHO cell line other than the glutamine synthetase (GS) gene expression system (GS-CHO expression system), the addition of 2-8 mM L-glutamine or a similar amount of GlutaMAX Supplement (Cat. No. 35050079, 35050061) is recommended.
Note: We recommend that you supplement with glucose as needed so that glucose levels don’t fall below 2g/L in culture (see the product User Guide for more details).
Some cell lines may require an adaptation period, and the culture may lag in growth until it becomes accustomed to GlutaMAX Supplement (Cat. No. 3050061, 35050079). For serum-free media it is best to add L-glutamine along with GlutaMAX supplement. We don’t recommend that you use GlutaMAX supplement with insect cells such as Sf9.
The formulation and manufacturing process are the same for all three products. The main difference is in the “intended use” statement. CTS GlutaMAX-I Supplement (Cat. No. A1286001) and CTS GlutaMAX Supplement (Cat. No. A4737001) have an intended use statement of “For Research Use or Manufacturing of Cell, Gene, or Tissue-Based Products. CAUTION: Not intended for direct administration into humans or animals”. GlutaMAX Supplement (Cat. No. 35050061) has an intended use statement of “For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals”. The catalog number to use will depend on the specific application.
Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.