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cDNA Synthesis Kits and Master Mixes

A variety of full kits and master mixes that facilitate first strand cDNA synthesis reactions and processes.
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1

Thermo Scientific™ RevertAid RT Reverse Transcription Kit

The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates.

2

Thermo Scientific™ Maxima H Minus Double-Stranded cDNA Synthesis Kit

The Thermo Scientific Maxima H Minus Double-Stranded cDNA Synthesis Kit is a complete system for efficient synthesis of double-stranded cDNA from total RNA or mRNA.

Reverse Transcriptase Maxima H Minus
Reaction Format Separate Components
Final Product Type Double Stranded cDNA
Product Type cDNA Synthesis Kit
Sample Type RNA
For Use With (Application) Cloning
Product Line Maxima
3

Thermo Scientific™ RevertAid H Minus First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

Shipping Condition Dry Ice
Format Kit
Reverse Transcriptase RevertAid H Minus
Size (Final Product) Up to 13 kb
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 42°C to 45°C
Technique Reverse Transcription
Starting Material RNA
For Use With (Application) Real Time PCR (qPCR),RT-PCR
4

Applied Biosystems™ TaqMan™ Reverse Transcription Reagents  DFS Item

TaqMan Reverse Transcription Reagents provide the necessary reagents for reverse transcriptase-PCR (RT-PCR). Random hexamers, oligo (dT)16, and reverse primers are included for cDNA synthesis flexibility.

Shipping Condition Dry Ice
Format Kit
Reverse Transcriptase MultiScribe
Size (Final Product) Up to 5 kb
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Concentration 50 U/μL
Starting Material RNA
For Use With (Application) Real Time PCR (qPCR)
5

Invitrogen™ SuperScript™ VILO™ cDNA Synthesis Kit  DFS Item

The SuperScript VILO cDNA Synthesis Kit is designed to generate first strand cDNA for two-step RT-qPCR applications. The kit is supplied in a two-tube format with the VILO reaction mix and an enzyme blend in separate tubes.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript III
Final Product Type First-Strand cDNA
For Use With (Application) Real Time PCR (qPCR)
Detection Method qPCR
Format Kit
Size (Final Product) Up to 10 kb
Reaction Format Master Mix
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
6

Thermo Scientific™ Maxima™ H Minus cDNA Synthesis Master Mix

Thermo Scientific Maxima H Minus cDNA Synthesis Master Mix, available with or without dsDNase, provides all cDNA synthesis reaction components in a convenient one-tube master mix.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase Maxima H Minus
Final Product Type First-Strand cDNA
Quantity Each
For Use With (Application) Real Time PCR (qPCR),RT-PCR
Format Master Mix
Size (Final Product) Up to 20 kb
Reaction Format Premixed Components
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material RNA
7

Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

Shipping Condition Dry Ice
Content And Storage

• RevertAid Reverse Transcriptase
• RiboLock RNase Inhibitor
• 5X Reaction Buffer
• dNTP Mix
• Oligo(dT)18 Primer
• Random Hexamer Primer
• Control GAPDH RNA
• Forward GAPDH Primer (10 μM)
• Reverse GAPDH Primer (10 μM)
• Nuclease-free water

Store at –20°C.
Reverse Transcriptase RevertAid
Final Product Type First-Strand cDNA
For Use With (Application) RT-PCR
Format Kit
Size (Final Product) Up to 13 kb
Reaction Format Separate components
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
8

Invitrogen™ SuperScript™ VILO™ Master Mix  DFS Item

SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase SuperScript III
Final Product Type First-Strand cDNA
Concentration 5X
For Use With (Application) Real Time PCR (qPCR)
Detection Method qPCR
Format Master Mix
Size (Final Product) Up to 10 kb
Reaction Format Master Mix
Reaction Speed 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 42°C
Technique Reverse Transcription
Starting Material RNA
9

Invitrogen™ SuperScript™ IV Template Switching RT Master Mix

Invitrogen SuperScript IV Template-Switching RT Master Mix is designed for high template switching efficiency in cDNA synthesis reactions.

GC-Rich PCR Performance High
Format Master Mix
Final Product Type First-Strand cDNA
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Technique Template Switching Reverse Transcription
Concentration 4X
Starting Material Cells or RNA
10

Invitrogen™ SuperScript™ III CellsDirect™ cDNA Synthesis Kit  DFS Item

The SuperScript III CellsDirect cDNA Synthesis Kit is optimized for synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating the RNA.

GC-Rich PCR Performance High
Format Kit
Reverse Transcriptase SuperScript III
Size (Final Product) Up to 4.5 kb
Reaction Format Separate components
Final Product Type First-Strand cDNA
Reaction Speed 50 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Reduced
Optimal Reaction Temperature 50°C
Technique Reverse Transcription
Starting Material Cells
For Use With (Application) Real Time PCR (qPCR)
11

Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit  DFS Item

The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes.

Shipping Condition Dry Ice
Format Kit
Reverse Transcriptase MultiScribe
Size (Final Product) Up to 7 kb
Reaction Format Premixed Components
Final Product Type First-Strand cDNA
Reaction Speed 30 to 60 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 37°C
Technique Reverse Transcription
Starting Material RNA
For Use With (Application) Real Time PCR (qPCR),RT-PCR
12

Thermo Scientific™ Maxima First Strand cDNA Synthesis Kit for RT-qPCR

The Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR, available with or without dsDNase, is a convenient system optimized for cDNA synthesis in two-step quantitative RT-PCR (RT-qPCR) applications.

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Reverse Transcriptase Maxima
Final Product Type First-Strand cDNA
Quantity Each
For Use With (Application) Real Time PCR (qPCR)
Format Kit
Size (Final Product) Up to 20 kb
Reaction Format Separate components
Reaction Speed 30 min.
Reagent Type Reverse Transcription
Ribonuclease H Activity Yes
Optimal Reaction Temperature 50°C to 55°C
Technique Reverse Transcription
Starting Material RNA
13

Invitrogen™ SuperScript™ Double-Stranded cDNA Synthesis Kit  DFS Item

Maximize the yield of full-length cDNA, as well as that of overall cDNA synthesis

Shipping Condition Wet Ice
Format Kit
Reverse Transcriptase SuperScript™ II
Final Product Type cDNA (Double-Stranded)
Product Type cDNA Synthesis Kit
Sample Type RNA
Labeling Method Direct Labeling
Optimal Reaction Temperature 42°C
For Use With (Application) Microarray Analysis
Product Line SuperScript
14

Invitrogen™ SuperScript™ First-Strand Synthesis System for RT-PCR  DFS Item

The SuperScript First-Strand Synthesis System for RT-PCR is optimized for the synthesis of first-strand cDNA from purified poly(A)+ or total RNA.

15

Invitrogen™ SuperScript™ IV CellsDirect™ Lysis Reagents  DFS Item

The SuperScript IV CellsDirect Lysis Reagents kit includes lysis solution, stop solution, and lysis enhancer. These reagents are part of the SuperScript IV CellsDirect cDNA Synthesis Kit and are made available separately here for use when processing a large number of samples.