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Analog of fluo-4 with lower Ca2+-binding affinity, making it suitable for detecting intracellular calcium levels in 1μM to 1mM range that would saturate esponse of fluo-3 and fluo-4
Rigorous methods for removing as much unconjugated dye as practical, followed by assay of dextran conjugates by thin-layer chromatography to help ensure absence of low molecular weight contaminants
The LysoLight Antibody Labeling Kits offer an efficient method for conjugating antibodies with LysoLight dyes. This process allows for specific and sensitive detection of internalized antibodies within lysosomes using fluorescence microscopy, flow cytometry, or high content imaging platforms.
Imaging dye that binds to free Ca2+. Fura-2 is the preferred dye for ratiometric imaging microscopy with digital image analysis, especially when altering excitation wavelengths is more practical than detecting multiple emission wavelengths.