Cell Function Reagents and Kits
Molecular Probes™ Fluo-4, AM, cell permeant
Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.
Invitrogen™ Fluorescein Diacetate (FDA)
Cell-permeant esterase substrate that can serve as viability probe
Invitrogen™ Calcium Calibration Buffer Kit #1, zero and 10 mM CaEGTA
Determine the dissociation constant (Kd) of fluorescent Ca2+ indicators using a fluorometer or quantitative imaging system
Invitrogen™ Amplex™ Red Catalase Assay Kit
For use with absorbance and fluorescence intensity techniques
Invitrogen™ Magnesium Green™, Pentapotassium Salt, cell impermeant
Exhibits higher affinity for Mg2+ (Kd approximately 1.0mM) than mag-fura-2 (Kd approximately 1.9mM) or mag-indo-1 (Kd approximately 2.7mM)
Invitrogen™ CyQUANT™ LDH Cytotoxicity Assays
CyQUANT cytotoxicity assays measure cellular toxicity based on the release of cytosolic enzymes (LDH or G6PD) from damaged cells. These cytotoxicity assays utilize either colorimetric or fluorescence detection and allow continuous monitoring of cell death using microplate readers.
Invitrogen™ Click-iT™ EdU Alexa Fluor™ 647 HCS Assay
Uses a highly specific click reaction
Invitrogen™ Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 555 dye
Fluorescent labeling of proliferating cells is accurate and compatible with antibody methods
Invitrogen™ RediPlate™ 96 EnzChek™ Tyrosine Phosphatase Assay Kit
Ready-to-use microplate assay
Invitrogen™ Newport Green™ DCF Diacetate, cell permeant
Has moderate zinc-binding affinity (Kd for Zn2+ approximately 1μM) but is essentially insensitive to Ca2+
Invitrogen™ SBFI, AM, cell permeant
Sodium-sensitive molecule used to estimate Na+ gradients in isolated mitochondria, to measure intracellular NA+ levels, or to measure Na+ efflux in cells
Invitrogen™ Rhod-5N, Tripotassium Salt, cell impermeant
Cells may be physically loaded with the cell-impermeant salt forms of these indicators using patch pipet, microinjection or pinocytotic cell-loading reagent
Invitrogen™ Rhod-2, Tripotassium Salt, cell impermeant
Cells may be physically loaded with cell-impermeant salt forms of these indicators using patch pipet, microinjection or pinocytotic cell-loading reagent
Invitrogen™ Epidermal Growth Factor, Biotinylated, complexed to Alexa Fluor™ 488 Streptavidin (Alexa Fluor™ 488 EGF complex)
The biotinylated conjugates utilize biotin-XX, which contains a long spacer arm designed to enhance the probe affinity for the EGF receptor
Invitrogen™ FluoZin™-2, AM, cell permeant
Designed to detect Zn2+ concentrations that are present in synaptic vesicles and released in response to electrical stimulation or excitotoxic agonists