Crosslinking Reagents
Thermo Scientific™ BS3-d4 (bis(sulfosuccinimidyl) 2,2,7,7-suberate-d4)
Crosslink amines to amines using these heavy (deuterated) and light matched pairs of an 11.4A, NHS-ester crosslinker for mass spectrometry analysis of protein interactions.
Thermo Scientific™ EGS (ethylene glycol bis(succinimidyl succinate))
Crosslink amines to amines with this membrane permeable crosslinker composed of NHS-ester groups and a 16.1A hydroxylamine-cleavable spacer arm.
Thermo Scientific™ SM(PEG)24 (PEGylated, long-chain SMCC crosslinker)
Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).
Thermo Scientific™ EMCH (N-ε-maleimidocaproic acid hydrazide)
Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this mid-length (11.8A) crosslinker containing maleimide and hydrazide groups.
Thermo Scientific™ DSS (disuccinimidyl suberate), No-Weigh™ Format
Crosslink amines to amines with disuccinimidyl suberate composed of NHS-ester groups with a membrane-permeable, mid-length spacer for intracellular protein crosslinking.
Thermo Scientific™ DMS (dimethyl suberimidate)
Crosslink amines to amines with dimethyl suberimidate (CAS 34490-86-3); composed of imidoester groups at either end of a membrane-permeable, 8-atom spacer arm.
Thermo Scientific™ Sulfo-GMBS (N-γ-maleimidobutyryl-oxysulfosuccinimide ester)
Crosslink amines to sulfhydryls with this short, water-soluble crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of an aliphatic, 6-atom (7.3A) spacer arm.
Thermo Scientific™ PEG12-SPDP (PEGylated, long-chain SPDP crosslinker)
Very long (54.1A) amine-to-sulfhydryl crosslinker; NHS-ester and pyridyldithiol groups at ends of 12-unit polyethylene glycol spacer arm; forms cleavable disulfide bonds; water-soluble.
Thermo Scientific™ SDA (NHS-Diazirine) (succinimidyl 4,4'-azipentanoate)
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).
Thermo Scientific™ PMPI (p-maleimidophenyl isocyanate)
Short (8.7A) hydroxyl-to-sulfhydryl crosslinker; isocyanate and maleimide groups; label -OH by urethane bonds for conjugation to cysteines by thioether bonds.
Thermo Scientific™ SDAD (NHS-SS-Diazirine) (succinimidyl 2-((4,4'-azipentanamido)ethyl)-1,3'-dithiopropionate)
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).
Thermo Scientific™ Sulfo-SDA (Sulfo-NHS-Diazirine) (sulfosuccinimidyl 4,4'-azipentanoate)
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).
Thermo Scientific™ TMEA (tris(2-maleimidoethyl)amine)
A 3-way, sulfhydryl-to-sulfhydryl crosslinker; tris (2-maleimidoethyl) amine; composed of three maleimide groups on short spacer arms; conjugate protein or peptide cysteines.
Thermo Scientific™ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weigh™ Format
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.
Thermo Scientific™ Sulfo-EGS (ethylene glycol bis(sulfosuccinimidyl succinate))
Crosslink amines to amines with a crosslinker composed of sulfo-NHS-ester groups and hydroxylamine-cleavable spacer arm; water-soluble for cell-surface protein crosslinking.