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  6. Thermo Scientific™ Pierce™ Premium Grade Sulfo-NHS (N-hydroxysulfosuccinimide)

Thermo Scientific™ Pierce™ Premium Grade Sulfo-NHS (N-hydroxysulfosuccinimide)

Catalog No. PIPG82072
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500 mg
10 g
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PIPG82072 10 g
PIPG82071 500 mg
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Catalog No. PIPG82072 Supplier Thermo Scientific™ Supplier No. PG82072

Description

Optimize applications where product integrity and risk minimization are paramount with our highest quality formulation of this N-hydroxysuccinimide modification reagent.

Thermo Scientific Pierce Premium Grade Sulfo-NHS is our highest quality formulation of this N-hydroxysuccinimide chemical modification reagent, specially characterized for applications where product integrity and risk minimization are paramount.

Features of Premium Grade Sulfo-NHS:

  • Sulfo-NHS—use with EDC crosslinker to prepare amine-reactive NHS ester reagents
  • High quality—identity and purity confirmed by several tests, including quantitative NMR
  • Product integrity—enhanced level of testing and characterization compared to standard grade
  • Lot retention—ample supply of past lots retained to ensure future process testing
  • Change management—Change Control Notification (CCN) service
  • Consistent manufacture—batch-specific manufacturing documentation review

Applications:

  • Improve efficiency of EDC coupling reactions
  • Convert carboxyls to amine-reactive sulfo-NHS esters
  • Membrane-impermable for cell surface labeling
  • Crosslink proteins to carboxyl-coated beads or surfaces more efficiently
  • Activate nanoparticles with amine-reactive sulfo-NHS esters

Compared to the standard grade product, Premium Grade Sulfo-NHS provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. Sulfo-NHS (N-hydroxysulfosuccinimide) enables control and modification of carbodiimide crosslinking reactions involving activation of carboxylates (—COOH) for conjugation with primary amines (—NH2). Derivatives are easily synthesized by mixing the sulfo-NHS with a carboxyl-containing molecule and a dehydrating agent such as the carbodiimide EDC (EDAC). The method is the basis for generating many types of protein labeling reagents, including amine-reactive fluorescent dyes, biotin affinity tags and pegylation compounds.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Products

  • Sulfo-NHS (N-hydroxysulfosuccinimide) (Cat. No. 24510)
  • Pierce™ Premium Grade EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) (Cat. No. PG82073)

Specifications

Chemical Reactivity Amine-Carboxyl
Cleavable No
Molecular Weight (g/mol) 217.13
PEGylated No
Content And Storage Upon receipt store at 4°C.
Cell Permeability No
Shipping Condition Ambient
Product Line Pierce
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety Carbodiimide
Spacer Short (<10 Å)
Form Powder
Quantity 10 g
Solubility Water
Format Standard, Single-use, Premium-grade
Water Soluble Yes
Product Type Crosslinker
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Frequently Asked Questions (FAQs)

Can you provide the shelf-life for Premium Grade Sulfo-NHS (N-hydroxysulfosuccinimide)?

Premium Grade Sulfo-NHS (N-hydroxysulfosuccinimide) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

For Research Use Only. Not for use in diagnostic procedures.