Crosslinking Reagents
Crosslinking Reagents
Thermo Scientificâ„¢ Pierceâ„¢ EDC, No-Weighâ„¢ Format
Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
Thermo Scientificâ„¢ BS3 (bis(sulfosuccinimidyl)suberate)
Crosslink amines to amines with BS3; composed of sulfo-NHS-ester groups separated by an 11.4A, water-soluble spacer arm that is useful for cell-surface protein crosslinking.
Thermo Scientificâ„¢ Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate), No-Weighâ„¢ Format
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker made of NHS-ester and maleimide groups at ends of a cyclohexane-containing, 8-atom spacer arm.
Thermo Scientificâ„¢ DSS (disuccinimidyl suberate), No-Weighâ„¢ Format
Crosslink amines to amines with disuccinimidyl suberate composed of NHS-ester groups with a membrane-permeable, mid-length spacer for intracellular protein crosslinking.
Thermo Scientificâ„¢ NHS-Phosphine
Derivatize proteins or amine surfaces for selective conjugation, detection or purification of azide-tagged targets with these activated phosphine compounds.
Thermo Scientificâ„¢ NHS-Azide
Activated azido compounds to derivatize proteins or amine surfaces for selective conjugation, detection or purification by phosphine-modified probes.
Thermo Scientificâ„¢ Sulfo-NHS (N-hydroxysulfosuccinimide), No-Weighâ„¢ Format
Prepare amine-reactive sulfo-NHS-ester crosslinkers or enhance carbodiimide (EDC) conjugation for peptides or proteins using N-hydroxysulfosuccinimide, CAS 106627-54-7.
Thermo Scientificâ„¢ Pierceâ„¢ SMCC
No-Weighâ„¢ Format
Thermo Scientificâ„¢ Pierceâ„¢ DSP, No-Weighâ„¢ Format
Crosslink amines to amines with dithiobis succinimidyl propionate, composed of NHS-ester groups and disulfide-containing, 12.0A spacer arm and cleavable with reducing agents.
Thermo Scientific Pierceâ„¢ Pierceâ„¢ SM(PEG)12, No-Weighâ„¢ Format
Thermo Scientificâ„¢ Pierceâ„¢ SM(PEG)12 is hetero-bifunctional crosslinker with N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules.
Thermo Scientific Pierceâ„¢ Pierceâ„¢ SM(PEG)2, No-Weighâ„¢ Format
Thermo Scientificâ„¢ Pierceâ„¢ SM(PEG)2 is hetero-bifunctional crosslinker with N-hydroxysuccinimide (NHS) ester and maleimide groups that allow covalent conjugation of amine- and sulfhydryl-containing molecules.
Thermo Scientificâ„¢ Mass Spectrometry Cleavable Crosslinkers
Improve sensitivity of mass spectrometry detection of crosslinked proteins with MS-cleavable DSSO and DSBU heterotrifunctional crosslinkers.
Thermo Scientificâ„¢ BS(PEG)5 (PEGylated bis(sulfosuccinimidyl)suberate)
Crosslink amines to amines with these long (21.7A and 35.8A) crosslinkers composed of NHS-ester groups and 5-unit or 9-unit PEG spacer arms that enhance protein solubility.