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Thermo Scientific™ LC-SDA (NHS-LC-Diazirine) (succinimidyl 6-(4,4'-azipentanamido)hexanoate)

Catalog No. PI26168
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PI26168 50 mg
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Catalog No. PI26168 Supplier Thermo Scientific™ Supplier No. 26168
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Description

Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).

Thermo Scientific™ Pierce NHS-Diazirine Crosslinkers combine proven NHS-ester and diazirine-based photoreaction chemistries to conjugate amine-containing molecules with nearly any other functional group via long-wave UV-light activation.

The succinimidyl-ester diazirine (SDA) reagents are a new class of crosslinkers that combine proven amine-reactive chemistry with an innovative and efficient diazirine-based photochemistry for conjugating amine-containing molecules to nearly any other functional group. The SDA crosslinkers include six compounds differing in spacer arm lengths, ability to cleave the crosslinked proteins, and presence or absence of a charged group for membrane permeability. Protein crosslinking is an important technique used to understand protein structure and to stabilize protein-protein interactions. Thermo Scientific SDA Reagents extend the efficiency and range of interactions that can be explored by this approach.

Highlights:

  • Versatile - different amine-reactive diazirine derivatives capture specific intracellular or cell-surface protein interactions

  • Controllable - two-step chemical crosslinking is activated using common laboratory UV lamps

  • Reversible - disulfide-containing spacers enable cleavage using reducing agents

  • Easy - reagents are photo-stable under typical laboratory lighting conditions so there is no need to perform experiments in the dark

  • Better than aryl azides - diazirine photo-reactive group has better photostability and is more efficiently activated by long-wave UV light

Specifications

Chemical Reactivity Amine-Nonselective
Cleavable No
Description LC-SDA
Molecular Weight (g/mol) 338.36
PEGylated No
Spacer Arm Length 12.5 Å
Content And Storage Upon receipt store product at 4°C protected from light and moisture.
Cell Permeability Yes
Shipping Condition Ambient
Product Line Pierce
Labeling Method Chemical Labeling
Crosslinker Type Heterobifunctional
Reactive Moiety NHS Ester, Diazirine
Spacer Medium (10 to 30 Å)
Form Solid
Quantity 50 mg
Solubility DMF, DMSO
Format Standard
Water Soluble No
Product Type Crosslinker
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For Research Use Only. Not for use in diagnostic procedures.