Miscellaneous Molecular Biology Reagents
Invitrogen™ UltraPure™ Dithiothreitol
UltraPure Dithiothreitol (DTT, Cleland's reagent), which reduces disulfides to their corresponding thiols, is used at low concentrations to stabilize enzymes containing free sulfhydryl groups.
Invitrogen™ UltraPure™ Formamide
UltraPure Formamide is commonly used for denaturing nucleic acids for sequencing gel electrophoresis, electron microscopy, and hybridization.
Invitrogen™ IPTG
IPTG (isopropylthio-β-galactoside), a structural non-metabolizable analogue of allolactose, is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-Gal or Bluo-Gal to detect lac gene activity in cloning procedures.
Invitrogen™ EDTA (0.5 M), pH 8.0, RNase-free
Invitrogen Molecular biology grade, 0.5 M EDTA, pH 8.0 solution is supplied in one bottle. The solution is certified RNase-free, economical, and ready-to-use.
Invitrogen™ Yeast tRNA (10 mg/mL)
Invitrogen Yeast tRNA purified from brewer's yeast. When used at a final concentration of 10 to 20 μg/mL, yeast tRNA is an effective coprecipitant to aid in recovery of small amounts of nucleic acids.
Invitrogen™ NaCl (5 M), RNase-free
Invitrogen Molecular biology grade, 5 M NaCl solution is supplied in one bottle. The solution is certified RNase-free, economical, and ready-to-use.
Invitrogen™ Sodium Acetate (3 M), pH 5.5, RNase-free
Invitrogen Molecular biology grade, 3M Sodium Acetate solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.
Invitrogen™ Ammonium Acetate (5 M), RNase-free
Invitrogen Molecular biology grade, 5 M Ammonium Acetate solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use.
Invitrogen™ Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1)
In RNA extraction procedures, Acid Phenol:Chloroform:IAA aids in the removal of DNA (it partitions into the organic phase), helps to stabilize the interface, and prevents foaming when mixing.
Invitrogen™ Salmon Sperm DNA, sheared (10 mg/mL)
Sheared Salmon Sperm DNA is a very effective blocking agent when used in Northern prehybridization and hybridization buffers at a concentration of 100 to 200 μg/mL.
Invitrogen™ DNase I Buffer (10X)
An Invitrogen component of the MAXIscript™ Kits, the buffer is supplied in one tube containing 1 mL.
Invitrogen™ MagMAX™ Wash Solution 2 Concentrate
Invitrogen MagMAX Wash Solution 2 Concentrate is a component of all the MagMAX Isolation Kits that may be purchased separately.
Invitrogen™ SDS, 20% Solution, RNase-free
Invitrogen molecular biology grade, SDS, 20% Solution is supplied in one bottle containing 250 mL.
MilliporeSigma™ Calbiochem™ SPUTOLYSIN™ Reagent
For isolation of epithelial cells, pathogenic or saprophytic bacteria, fungi, and yeasts from sputum
Polysorbate 80
CAS: 9005-65-6 Synonym: Polyoxyethylene(20)sorbitan monooleate