Miscellaneous Molecular Biology Reagents
Invitrogen™ UltraPure™ Guanidine Isothiocyanate Solution
UltraPure Guanidine Isothiocyanate Solution consists of 4M guanidine isothiocyanate, 50 mM Tris-HCl (pH 7.5), 25 mM EDTA.
Invitrogen™ UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)
UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl.
Invitrogen™ UltraPure™ Buffer-Saturated Phenol
UltraPure™ Buffer-Saturated Phenol is used in the purification of nucleic acids. The reagent, which consists of UltraPure™ Phenol that has been saturated with Tris-HCl buffer, is already buffer equilibrated to pH >7.4.
Invitrogen™ UltraPure™ Glycerol
UltraPure Glycerol is suitable for the concentration and storage of enzymes at low temperatures. A 50% (v/v) solution will not freeze at -20°C.
Invitrogen™ IPTG
IPTG (isopropylthio-β-galactoside), a structural non-metabolizable analogue of allolactose, is an inducer of β-galactosidase activity in bacteria and is suitable for use with X-Gal or Bluo-Gal to detect lac gene activity in cloning procedures.
Invitrogen™ X-Gal
X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) is a histochemical substrate for β-galactosidase. This substrate yields a blue precipitate upon hydrolysis, making it suitable for use in immunoblotting or immunocytochemical assays.
Invitrogen™ UltraPure™ Formamide
UltraPure Formamide is commonly used for denaturing nucleic acids for sequencing gel electrophoresis, electron microscopy, and hybridization.
Invitrogen™ Guanidine Isothiocyanate
UltraPure Guanidine Isothiocyanate is a strong protein denaturant when used at high concentrations.
MilliporeSigma™ Calbiochem™ Tetrahydrouridine
Potent competitive inhibitor of cytidine deaminase
Thermo Scientific Chemicals Dodecyl sulfate, sodium salt, 99%, for biochemistry
CAS: 151-21-3 Formule moléculaire: C12H25NaO4S,C12H25NaO4S
Polysorbate 80
CAS: 9005-65-6 Synonyme: Polyoxyethylene(20)sorbitan monooleate
Invitrogen™ EDTA (0.1 mM), pH 8.0, RNase-free
Invitrogen 0.1 mM EDTA is a commonly recommended storage solution for RNA samples. 0.1 mM EDTA is in nuclease-free, ultrapure water. Supplied in one bottle of 50 mL.
Invitrogen™ Guanidine Thiocyanate
Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent. It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents.
Invitrogen™ MagMAX™ Wash Solution 2 Concentrate
Invitrogen MagMAX Wash Solution 2 Concentrate is a component of all the MagMAX Isolation Kits that may be purchased separately.
Invitrogen™ IPTG (Dioxane-Free)
Invitrogen IPTG (Isopropyl-beta-D-thiogalactoside) is an inducer of the lac operon and is frequently used in conjunction with X-GAL to determine the lac phenotype of recombinant plasmids or phage.