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Modifying Enzymes

Proteins that have the ability to structurally alter macromolecules without being altered themselves. Useful for a variety of molecular biology procedures; products include DNA and RNA polymerases, phosphatases, kinases, nucleases, etc.
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Fisher BioReagents™ Proteinase K (Tritirachium album/Molecular Biology)

EDGE
Content And Storage -20°C
Form Solid
Product Type Proteinase K
Color White
For Use With (Application) Isolation of DNA and RNA from cell and tissue preparations, inactivating many Enzymes, including Endogenous Nucleases
Source Tritirachium album
2

Thermo Scientific™ T4 Polynucleotide Kinase (10 U/μL)

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer of the gamma-phosphate from ATP to the 5'-OH group of oligonucleotides, ss and dsDNA and RNA.

Product Type T4 Polynucleotide Kinase
Enzyme T4 Polynucleotide Kinase
Compatible Buffer 10X Reaction Buffer
Concentration 10 U/μL
3

Thermo Scientific™ Proteinase K, recombinant, PCR grade

Degrade proteins even in the presence of detergents with Proteinase K, a broad-range endolytic protease widely used for digestion of proteins in nucleic acid preparations.

Form Liquid
Product Type Proteinase K
4

Invitrogen™ Proteinase K Solution (20 mg/mL), RNA grade  DFS Item

Proteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.

5

MP Biomedicals™ Hyaluronidase, Bovine Testes

Bovine Testes

Content And Storage -20°C
Inhibitors Fe2+ and Fe3+ are inhibitory as are Mn2+ and Cu2+
Form Lyophilized Powder
Product Type Hyaluronidase
Molecular Weight (g/mol) ≈55
Color Tan
pH 4.5 to 6
Concentration ≥300 U/mg
For Use With (Application) Drug dispersion and delivery
Source Bovine testes
6

Invitrogen™ Ambion™ Recombinant RNase A  DFS Item

Rigorously tested for contaminating nonspecific endonuclease, exonuclease, and protease activity

7

Invitrogen™ LR Clonase™ II Plus enzyme  DFS Item

Mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor), and Xis (Excisionase) enzymes.

Shipping Condition Dry Ice
Content And Storage Includes LR Clonase II Plus enzyme and proteinase K solution (2 μg/μL). Store at -80°C. Guaranteed stable for 6 months when properly stored.
Product Type LR Clonase Enzyme
Enzyme LR Clonase
Compatible Buffer Reaction Buffer
Product Line Clonase, Gateway
8

Thermo Scientific™ Endonuclease IV, E.coli (2 U/μL)

Cut oxidative damaged DNA at AP sites with this enzyme, which recognizes apurinic/apyrimidinic sites and cleaves the phosphodiester bond 5' to the lesion.

9

MilliporeSigma™ Calbiochem™ Lysozyme, Chicken Egg White

Inhibits biological activity of lipopolysaccharides from periodontopathic bacteria

Content And Storage -20°C
Form Lyophilized Solid
Product Type Lysozyme, Chicken Egg White
Color Off-white
Source Chicken egg
10

Thermo Scientific™ DNase I, RNase-free (supplied with MnCl2) (1 U/μL)

Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.

11

Thermo Scientific™ Lambda Exonuclease (10 U/μL)

Lambda Exonuclease is a highly processive exodeoxyribonuclease that selectively digests the 5'-phosphorylated strand of double-stranded DNA in 5'-3' direction.

Product Type Exonuclease
Enzyme Exonuclease
Concentration 10 U/μL
12

Thermo Scientific™ Exonuclease I (20 U/μL)

Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.

Product Type Exonuclease I
Enzyme Exonuclease
Concentration 20 U/μL
13

MilliporeSigma™ Calbiochem™ β1-2,3,4,6-N-Acetylglucosaminidase

Catalyzes hydrolysis of β1-2,3,4,6-linked N-acetylglucosamine from complex carbohydrates