Modifying Enzymes

Fisher BioReagents™ Proteinase K (Tritirachium album/Molecular Biology)

Fisher Scientific Edge
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Thermo Scientific™ T4 DNA Ligase, LC (1 U/μL)

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.

Thermo Scientific™ E. coli DNA Ligase

Thermo Scientific™ E. coli DNA Ligase is an NAD-dependent DNA ligase. It catalizes formation of phosphodiester bonds between 5'-phospate and 3'-hydroxyl termini in double-stranded DNA.

Thermo Scientific Chemicals Pectinase, Aspergillus niger

Used in plant protoplast preparation to digest cell wall prior to organelle isolation. Pectinase helps facilitate the maceration of plant materials and extraction processes including organelle preparation.

MilliporeSigma™ RNase A

As supplied with Montage™ Plasmid Miniprep_HTS 96 Kit

Invitrogen™ Terminal Deoxynucleotidyl Transferase, recombinant  

Performance and quality tested

Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase), 20 U/μL  

Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA

Invitrogen™ Ribonuclease H  

Specifically degrades RNA strand of RNA-DNA hybrid to produce 5' phosphate-terminated oligoribonucleotides and single-stranded DNA

Invitrogen™ TURBO DNase™ (2 U/μL)  

Efficiently degrades and digests DNA

Invitrogen™ Terminal Deoxynucleotidyl Transferase (TdT) Buffer  

Supplied with Terminal Deoxynucleotidyl Transferase (TdT)

Invitrogen™ LR Clonase™ II Plus enzyme  

Mix contains a proprietary blend of Int (Integrase), IHF (Integration Host Factor), and Xis (Excisionase) enzymes.

MilliporeSigma™ Calbiochem™ RNase A, Bovine Pancreas

For selective degradation of RNA

Invitrogen™ Recombinant Proteinase K Solution (20 mg/mL)  

Recombinant Proteinase K is a nonspecific serine protease that is useful for general digestion of proteins.

MilliporeSigma™ Calbiochem™ Highly Purified Protease-Free RNase A, Bovine Pancreas

For selective degradation of RNA

Thermo Scientific™ T4 DNA Ligase, HC (30 U/μL)

T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.