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Dissociation Reagents

Reagents designed to dissociate cells from surface substrates and from other cells; suitable for use with tissues and adherent cells; various types and strengths available.
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1

Gibco™ CTS™ TrypLE™ Select Enzyme  DFS Item
GREENER_CHOICE

Gibco CTS TrypLE Select is a recombinant enzyme capable of dissociating a wide range of adherent mammalian cells.

2

Cytiva HyClone™ Trypsin Protease

Derived from porcine pancreas. HyClone™ Trypsin Protease is used to disaggregate adherent cells from substrate or surface to which they are attached.

Content And Storage Store frozen
Sterilization Method 0.1 μm Sterile-filtered
Form Liquid
Shelf Life 24 Months
Source Porcine pancreas
4

Corning™ Accutase™ Cell Detachment Solution, Liquid

Efficient, with gentle detachment of adherent cells within minutes

Content And Storage Store at -20°C.
Form Liquid
Formulation 1 X Accutase enzymes prepared in Dulbeccos PBS (0.2g/L KCl, 0.2g/L KH2PO5, 8g/L NaCl, and 1.15g/L Na2HPO4) containing 0.5 mm EDTA·4Na and 3 mg/L Phenol Red.
Sterility Sterile
5

Corning™ 0.25% Trypsin in HBSS w/o Calcium and Magnesium

Enzymatic dissociation reagents tested for porcine parvovirus

6

MilliporeSigma™ PluriSTEM™ Dispase-II Solution

a rapid and effective, yet gentle, agent for separating many tissues and cells grown in vitro.

7

Gibco™ Trypsin-EDTA (0.05%), phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas.

Chelators EDTA
Sterilization Method Sterile-filtered
With Additives Phenol Red,EDTA
Product Type Cell Culture Dissociation Reagent
Form Liquid
Tested For In vitro Bioassay
Classification Animal Origin
Shelf Life 24 Months
Osmolality 270 - 310 mOsm/kg
Sterility Sterile-filtered
Source Animal Origin
Product Line Gibco
Cell Type Mammalian Cells
8

MilliporeSigma™ Chemicon™ Accutase™ Cell Detachment Solution

useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

9

Gibco™ CTS™ Versene™ Solution  DFS Item

CTS Versene Solution is a cGMP-manufactured EDTA solution for use as a gentle non-enzymatic cell dissociation reagent.

10

Gibco™ CTS™ TrypLE™ Select Enzyme  DFS Item
GREENER_CHOICE

TrypLE™ Select CTS™, a non-animal alternative for porcine Trypsin, is a recombinant enzyme derived from microbial fermentation.

11

MilliporeSigma™ Trypsin 0.25%

The Trypsin 0.25%, In Hank's Balanced Salt Solution, without Ca 2+ and Mg 2+ is available in a 100mL format.

12

MilliporeSigma™ ESGRO Complete™ Accutase™ Cell Detachment Solution

a cell detachment solution of proteolytic and collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium.

13

Corning™ CellStripper Dissociation Reagent

Non-enzymatic cell dissociation solution gently dislodges adherent cells in culture

14

Corning™ 0.05% Trypsin/0.53mM EDTA in HBSS w/o Calcium and Magnesium

Used to enzymatically release adherent cells from tissue culture plates for passaging.

15

Corning™ 0.25% Trypsin, 0.1% EDTA in HBSS w/o Calcium, Magnesium and Sodium Bicarbonate

Used to enzymatically release adherent cells from tissue culture plates for passaging.