ELISA Substrates and Reagents
Invitrogen™ ELISA Buffer Kit
Concentrated
Invitrogen™ InstantOne ELISA Cell Lysis Buffer (5X)
Combine with Enhancer Solution (cat. IOES1) to make the 5x Cell Lysis Mix used in the InstantOne phospho ELISA kits. The 5x Cell Lysis Buffer contains a combination of detergents, phosphatase inhibitors, salts, and buffers. The Cell Lysis Mix can be applied to many cells and tissues.
Thermo Scientific™ QuantaBlu™ NS/K Fluorogenic Substrate Kit
Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.
Invitrogen™ CSPD™ Substrate (0.25 mM Ready-To-Use) with Nitro-Block™ Enhancer
Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.
Thermo Scientific™ ELISA Blocker Blocking Buffer
Increases signal-to-noise ratio and minimizes background
Thermo Scientific™ ELISA Wash Buffer (30X)
Concentrated wash buffer for efficient use of storage in the lab
Thermo Scientific™ OPD (o-phenylenediamine dihydrochloride)
Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.
Invitrogen™ DynaLight™ Substrate with RapidGlow™ Enhancer
A ready-to-use chemiluminescent substrate formulation optimized to achieve faster results in solution-based immunoassays. Thermo Scientific DynaLight Substrate with RapidGlow Enhancer provides a robust chemiluminescent reaction when catalyzed by alkaline
Invitrogen™ Assay Buffer (10X)
Used in immunoassays and in Western and Southern blotting for alkaline phosphatase Tropix™ substrate conjugates.
Invitrogen™ ELISA Stop Solution
A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution.
Invitrogen™ Luminescence Enhancer For Solution Assays, Sapphire-II™ Enhancer
Chemiluminescence enhancer that significantly reduces aqueous quenching and tune the wavelength of light emission.
Invitrogen™ CDP-Star™ Substrate (0.25 mM Ready-To-Use) with Nitro-Block-II™ Enhancer
Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.
Invitrogen™ Luminescence Enhancer For Solution Assays, Emerald-II™ Enhancer
The Emerald-II chemiluminescence enhancer significantly reduces aqueous quenching and tunes the wavelength of light emission.
Invitrogen™ Super AquaBlue ELISA Substrate
Enhanced ABTS substrate solution for ELISA which provides high signal and sensitivity, low background. Safe, stable and convenient to use. Oxidized by HRP-labeled probes, resulting in soluble, blue-green colored end product, whose absorption can be measured by spectrophotometers at 405nm.
Invitrogen™ Wash Buffer (25X)
Suitable for use with all ELISA assays