ELISA Substrates and Reagents
Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions
Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.
Thermo Scientific™ OPD Substrate Tablets (o-phenylenediamine dihydrochloride)
Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.
Thermo Scientific™ ONPG (o-nitrophenyl-β-D-galactopyranoside)
Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.
Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)
Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.
Thermo Scientific™ Pierce™ Stable Peroxide Substrate Buffer (10X)
Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.
Invitrogen™ CDP-Star™ Substrate (0.25 mM Ready-To-Use) with Nitro-Block-II™ Enhancer
Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.
Invitrogen™ ELISA Buffer Kit
Concentrated
Invitrogen™ Luminescence Enhancer For Solution Assays, Emerald-II™ Enhancer
The Emerald-II chemiluminescence enhancer significantly reduces aqueous quenching and tunes the wavelength of light emission.
Thermo Scientific™ QuantaBlu™ Fluorogenic Peroxidase Substrate Kit
Conduct stopped, non-stopped and kinetic assays using this soluble fluorogenic (chemifluorescent) peroxidase substrate for ELISA.
Thermo Scientific™ 1-Step™ ABTS Substrate Solution
Ready-to-use, water-soluble peroxidase substrate tablets that yield a measurable green end-product for use in ELISA methods.
Invitrogen™ Galacton-Star™ Substrate (50X Concentrate)
Concentrated chemiluminescent substrate for E.coli β-galactosidase assays. Thermo Scientific Galacton-Star Substrate (50X Concentrate) is highly sensitive which makes it ideal for detection of weak expression and for transfection normalization with o
Thermo Scientific™ Stop Solution for TMB Substrates
Stop TMB-based ELISA detection with this 0.16M sulfuric acid for use with the ELISA substrate 3,3',5,5' - tetramethylbenzidine (TMB).
Invitrogen™ cAMP-Screen™ Assay/Lysis Buffer
For use with the cAMP-Screen or cAmp-Screen Direct™ Immunoassay System.
Invitrogen™ Galacto-Light™ Reaction Buffer Diluent with Galacton-Plus™ Substrate
Established system with extended light emission and high sensitivity for reporter gene assays. Thermo Scientific Galacto-Light Reaction Buffer Diluent and the Galacton-Plus chemiluminescent substrate solution are used with the Galacto-Light Plus Reporter
Thermo Scientific™ ELISA Wash Buffer (30X)
Concentrated wash buffer for efficient use of storage in the lab