ELISA Substrates and Reagents
Thermo Scientific™ OPD Substrate Tablets (o-phenylenediamine dihydrochloride)
Water-soluble horseradish peroxidase (HRP) substrate that produces a yellow-orange product detectable at 492nm by ELISA plate readers.
Thermo Scientific™ 1-Step™ TMB ELISA Substrate Solutions
Thermo Scientific™ Pierce TMB ELISA Substrates detect horseradish peroxidase (HRP) activity, yielding a blue color (Amax = 370 nm and 652 nm) that changes to yellow (Amax = 450 nm) upon addition of a sulfuric or phosphoric acid stop solution.
Thermo Scientific™ Pierce™ Stable Peroxide Substrate Buffer (10X)
Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.
Thermo Scientific™ Pierce™ Diethanolamine Substrate Buffer (5X)
Optimized, reliable and convenient stock solutions of peroxide for HRP-based assays and diethanolamine for alkaline phosphatase (alk-phos) detection.
Thermo Scientific™ SuperSignal™ ELISA Femto Substrate
HRP substrate formulated for superior protein detection and low-end linearity in chemiluminescent ELISA applications.
Invitrogen™ CSPD™ Substrate (0.4 mM Ready-To-Use) with Sapphire-II™ Enhancer, Size B
Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.
Invitrogen™ ELISA Stop Solution
A ready-to-use solution intended for use as a stop solution in ELISA applications that use horseradish peroxidase and TMB substrate solution.
Invitrogen™ eBioscience™ ELISA/ELISPOT Diluent (5X)
Supplemented with fetal bovine serum as source of animal serum proteins. Used for blocking non-specific binding of ELISA plates, diluting recombinant standards and test samples and making the working dilutions of secondary detection reagent. Also contains 1% ProClin 150 as a preservative
Thermo Scientific™ ONPG (o-nitrophenyl-β-D-galactopyranoside)
Preferred colorimetric beta-galactosidase (b-Gal) substrate for ELISA applications.
Invitrogen™ eBioscience™ ELISA Wash Buffer, 1L packets
Suitable for washing ELISA plate microwells. eBioscience™ ELISA Wash Buffer is supplied in 10 packets of concentrated powder, yielding a total of 10 liters of 1x Wash Buffer.
Invitrogen™ Super AquaBlue ELISA Substrate
Enhanced ABTS substrate solution for ELISA which provides high signal and sensitivity, low background. Safe, stable and convenient to use. Oxidized by HRP-labeled probes, resulting in soluble, blue-green colored end product, whose absorption can be measured by spectrophotometers at 405nm.
Invitrogen™ InstantOne ELISA Cell Lysis Buffer (5X)
Combine with Enhancer Solution (cat. IOES1) to make the 5x Cell Lysis Mix used in the InstantOne phospho ELISA kits. The 5x Cell Lysis Buffer contains a combination of detergents, phosphatase inhibitors, salts, and buffers. The Cell Lysis Mix can be applied to many cells and tissues.
Thermo Scientific™ ELISA Blocker Blocking Buffer
Increases signal-to-noise ratio and minimizes background
Invitrogen™ Assay Buffer (10X)
Used in immunoassays and in Western and Southern blotting for alkaline phosphatase Tropix™ substrate conjugates.
Invitrogen™ CDP-Star™ Substrate (0.25 mM Ready-To-Use) with Nitro-Block-II™ Enhancer
Ready-to-use chemiluminescent alkaline phosphatase substrate, for protein or nucleic acid blotting on nitrocellulose membranes or solution-based assays.