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TrypLE Express Enzyme (1X), No Phenol Red DFS Item

Unlike porcine trypsin, free of animal-derived components

Manufacturer: life technologies  12604054

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Catalog No. 12604054

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Description & Specifications


Concentration 1X
Format Liquid
Kit Contents 1 universal bag(s)
Quantity 5L
Solution Type Trypsin Replacement
With Additives EDTA

Cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. Used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells.

  • Gentle on cells
  • Room temperature-stable for 24 months
  • Easy to use
  • Animal origin-free
  • Gentle on cells, purity increases specificity due to the action of a single enzyme and reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts
  • Can be directly substituted for trypsin in existing protocols
  • Dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS

Energy efficient, less hazardous, sustainable packaging

Cell Culture, Mammalian Cell Culture