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Self-coating synthetic surface for stem cells
Measure results in microplate assays or using flow cytometry
Cleavage produces blue-fluorescence
Used as substrate for fluorogenic assay of plasma and glandular kallikreins. MilliporeSigma™; Calbiochem™ Z-Phe-Arg-7-amido-4-methylcoumarin, Hydrochloride also acts as substrate for cathepsin B, cathepsin L, and papain.
The galactosidase substrate, DDAO galactoside yields a hydrolysis product that can be excited with the 633 nm laser (excitation/emission maxima ∼645/660).
Continuously assay acid phosphatases at low pH
Shipping Condition | Room Temperature |
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Excitation/Emission | 358⁄455 |
Substrate | Phosphatase Substrate |
Substrate Properties | Chemical Substrate |
Content And Storage | Store in freezer (-5 to -30°C). |
For Use With (Equipment) | Microplate Reader |
Form | Powder |
Label or Dye | DiFMU |
Color | Blue |
Target Enzyme | Phosphatases |
Detection Method | Fluorescence |
Easily distinguish substrate and hydrolysis product
Hydrolysis of substrate yields brightly fluorescent product