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Gene Reporter Systems

Reporter genes are attached to a regulatory sequence of a gene of interest. When expressed by the host organism, they generate measurable, easily identifiable characteristics that can be used as an indication of whether a gene has been taken up or expressed.
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1

Invitrogen™ Alexa Fluor™ 488 Reactive Dye Decapack, for microarrays  DFS Item

Matches the imaging channels of most popular microarray scanners

2

Invitrogen™ BLOCK-iT™ Lentiviral Pol II miR RNAi Expression System with EmGFP  DFS Item

Combines Invitrogen's BLOCK-iT Pol II miR RNAi and ViraPower™ Lentiviral technologies to facilitate creation of a replication-incompetent lentivirus that delivers a microRNA (miRNA) sequence of interest to dividing or non-dividing mammalian cells for RNA interference (RNAi) analysis

3

Invitrogen™ BLOCK-iT™ Pol II miR RNAi Expression Vector Kit  DFS Item

Combines the advantages of traditional RNAi vectors (stable expression and the ability to use viral delivery) with capabilities for tissue-specific expression and multiple target knockdown from the same transcript

4

Invitrogen™ BLOCK-iT™ Lentiviral Pol II miR RNAi Expression System  DFS Item

Combines Invitrogen's BLOCK-iT Pol II miR RNAi and ViraPower™ Lentiviral technologies to facilitate creation of a replication-incompetent lentivirus that delivers a microRNA (miRNA) sequence of interest to dividing or non-dividing mammalian cells for RNA interference (RNAi) analysis

5

Invitrogen™ Luc-Screen™ Extended-Glow Luciferase Reporter Gene Assay System  DFS Item

Thermo Scientific Luc-Screen Extended-Glow Luciferase Reporter Gene Assay System with extended-glow light emission is designed for sensitive detection of firefly luciferase reporter enzyme in microplate cell cultures. A linear signal is obtained with the

Méthode de détection Chemiluminescence
À utiliser avec (équipement) Luminometer (Microplate)
Substrat Luciferin
Type de produit Luciferase Reporter Gene Assay System
Enzyme cible Luciferase
Type d’échantillon Cell Cultures
Forme Liquid
À utiliser avec (application) Enzyme Assay
Cellules compatibles Mammalian Cells
Essai Luciferase Assay
Compatibilité à haut débit High-throughput Compatible
Propriétés de substrat Chemical Substrate
Affichage End Point
Type de substrat Luciferase Substrate
Gamme de produit Luc-Screen, NovaBright
6

Invitrogen™ λ DNA (cIind 1 ts857 Sam 7)  DFS Item

λ DNA (cIind 1 ts857 Sam 7) is isolated from a dam+ dcm+ E coli LE597 lysogen

7

Invitrogen™ NovaBright™ Phospha-Light™ EXP Assay Kit for SEAP (Secreted Placental Alkaline Phosphatase) Reporter Gene Detection  DFS Item

Chemiluminescent reporter gene assay for the ultrasensitive detection of secreted placental alkaline phosphatase or the non-secreted placental isozyme PLAP.

Compatibilité à haut débit High-throughput Compatible
Méthode de détection Enhanced Chemiluminescence
Propriétés de substrat Chemical Substrate
Format 96-well Plate
À utiliser avec (équipement) Luminometer (Microplate)
Substrat CSPD™
Type de produit Phosphatase Assay
Enzyme cible Placental Alkaline Phosphatase
Type de substrat AP (Alkaline Phosphatase) Substrate
Gamme de produit NovaBright
Contenu et stockage Component solution containing CSPD™ substrate and chemiluminescence enhancer (Store at 2°C to 6°C), placental alkaline phosphatase positive control (Store at ≤ -20°C)
8

Invitrogen™ BLOCK-iT™ Lentiviral RNAi Zeo Gateway™ Vector Kit  DFS Item

For lentiviral delivery and genomic integration of DNA coding for shRNA

9

Invitrogen™ RNA Fragmentation Reagents  DFS Item

For the fragmentation of aRNA prior to hybridization with oligonucleotide arrays

10

MilliporeSigma™ Calbiochem™ Coelenterazine

A cell-permeable aequorin luminophore that acts as a very sensitive and specific chemiluminescence probe of the superoxide anion.

12

Invitrogen™ Biotin-14-dCTP  DFS Item

Used for DNA biotinylation, an excellent, and sometimes superior alternative to conventional radioisotopic labeling

13

Invitrogen™ Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells  DFS Item

Combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.

Méthode de détection Chemiluminescence
Étiquette ou colorant Unconjugated
À utiliser avec (équipement) Luminometer (Microplate)
Substrat Galacton-Star™
Type de produit β-Galactosidase Reporter Gene Assay System
Enzyme cible Beta-Galactosidase
Type d’échantillon Cell Cultures
Forme Liquid
À utiliser avec (application) Enzyme Assay
Cellules compatibles Mammalian Cells
Essai β-Gal (lacZ) Assay
Compatibilité à haut débit High-throughput Compatible
Propriétés de substrat Chemical Substrate
Affichage End Point
Type de substrat Beta-Gal Substrate
Gamme de produit Gal-Screen, NovaBright
14

Invitrogen™ MessageAmp™ II aRNA Amplification Kit  DFS Item

Amplifies as little as 100pg of input RNA for GeneChip™ analysis

Format Kit
Type de produit aRNA Amplification Kit
Type d’échantillon Poly(A+) RNA, Total RNA
Comprend une étiquette ou un colorant No
Transcriptase inverse ArrayScript™
Gamme de produit Ambion, MessageAmp
15

Invitrogen™ CytoTune™ EmGFP Sendai Fluorescence Reporter  DFS Item

The CytoTune™ EmGFP Sendai Fluorescence Reporter helps you determine whether the Sendai particles that are used in all CytoTune™-iPS Sendai reprogramming kits can transduce into a given cell type before you advance to one of these kits.