Crosslinking Reagents
Thermo Scientific™ DSS (disuccinimidyl suberate), No-Weigh™ Format
Crosslink amines to amines with disuccinimidyl suberate composed of NHS-ester groups with a membrane-permeable, mid-length spacer for intracellular protein crosslinking.
Thermo Scientific™ DTSSP (3,3'-dithiobis(sulfosuccinimidyl propionate))
Crosslink amines to amines with Sulfo-DSP; composed of sulfo-NHS-ester groups and disulfide spacer arm (12.0A) that is cleavable with reducing agents.
Thermo Scientific™ Pierce™ DSP, No-Weigh™ Format
Crosslink amines to amines with dithiobis succinimidyl propionate, composed of NHS-ester groups and disulfide-containing, 12.0A spacer arm and cleavable with reducing agents.
Thermo Scientific™ SIA (succinimidyl iodoacetate)
Crosslink amines to sulfhydryls with this very short crosslinker composed of NHS-ester and iodoacetyl (haloacetyl) groups at ends of a 3-atom (1.5A) spacer arm.
Thermo Scientific™ BS(PEG)5 (PEGylated bis(sulfosuccinimidyl)suberate)
Crosslink amines to amines with these long (21.7A and 35.8A) crosslinkers composed of NHS-ester groups and 5-unit or 9-unit PEG spacer arms that enhance protein solubility.
Thermo Scientific™ Sulfo-SMPB (sulfosuccinimidyl 4-(N-maleimidophenyl)butyrate)
Crosslink amines to sulfhydryls with this mid-length, water-soluble crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of an aromatic, 10-atom spacer arm.
Thermo Scientific™ SPDP (succinimidyl 3-(2-pyridyldithio)propionate)
Crosslink amines to sulfhydryls with this crosslinker composed of NHS-ester and pyridyldithiol groups on 3-atom spacer arm; forms cleavable disulfide bonds with cysteines.
Thermo Scientific™ DST (disuccinimidyl tartrate)
Crosslink amines to amines with disuccinimidyl tartrate, composed of NHS-ester groups and a diol-containing spacer arm (6.4A) that is periodate-cleavable.
Thermo Scientific™ SBAP (succinimidyl 3-(bromoacetamido)propionate)
Crosslink amines to sulfhydryls with this short crosslinker composed of NHS-ester and bromoacetyl (haloacetyl) groups at ends of a 7-atom (6.2A) spacer arm.
Thermo Scientific™ MPBH (4-(4-N-maleimidophenyl)butyric acid hydrazide)
Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this long (17.9A) crosslinker containing maleimide and hydrazide groups.
Thermo Scientific™ AMAS (N-α-maleimidoacet-oxysuccinimide ester)
Crosslink amines to sulfhydryls with this very short crosslinker composed of NHS-ester and maleimide groups at ends of an aliphatic, 4-atom (4.4A) spacer arm.
Thermo Scientific™ PDPH (3-(2-pyridyldithio)propionyl hydrazide)
Crosslink sulfhydryls to periodate-treated glycoprotein sugars via cleavable disulfide bonds using this short crosslinker containing pyridyldithiol and hydrazide groups.
Thermo Scientific™ SM(PEG)8 (PEGylated, long-chain SMCC crosslinker)
Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).
Thermo Scientific™ Pierce™ Premium Grade Sulfo-SMCC (sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate)
Optimize applications where product integrity and risk minimization are paramount our highest quality formulation of this popular amine-to-sulfhydryl crosslinker.
Thermo Scientific™ SDAD (NHS-SS-Diazirine) (succinimidyl 2-((4,4'-azipentanamido)ethyl)-1,3'-dithiopropionate)
Very short to mid-length (3.9 to 13.5A), amine-to-anything crosslinkers; NHS-ester and diazirine reactive groups; photoactivate with UV light (Amax 330 to 370nm).