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Immobilize or conjugate proteins using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (CAS 25952-53-8), a water-soluble, carboxyl-to-amine crosslinker.
Description | EDC |
---|---|
Méthode d’étiquetage | Chemical Labeling |
Perméabilité cellulaire | No |
Cleavable | No |
Type de produit | Crosslinker |
Longueur du bras espaceur | 0.0 Å |
Réactivité chimique | Amine-Carboxyl |
Conditions d’expédition | Wet Ice |
Forme | Powder |
Contenu et stockage | Upon receipt store desiccated at -20°C. |
Pégylé | No |
Entretoise | Short (<10 Å) |
Poids moléculaire | 191.7 |
Format | Standard, Single-use, Premium-grade |
Type d’agent de réticulation | Heterobifunctional |
Solubilité | Water |
Hydrosoluble | Yes |
Groupement de réactifs | Carbodiimide |
Used to label E.coli thioredoxin.
Crosslink amines to amines with disuccinimidyl tartrate, composed of NHS-ester groups and a diol-containing spacer arm (6.4A) that is periodate-cleavable.
Crosslink amines to amines with dimethyl suberimidate (CAS 34490-86-3); composed of imidoester groups at either end of a membrane-permeable, 8-atom spacer arm.
Crosslink amines to sulfhydryls with this long crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of an aliphatic, 13-atom (16.3A) spacer arm.
Crosslink sulfhydryls to sulfhydryls (cysteine residues) with this long, pegylated, water-soluble crosslinker composed of maleimide groups and 3-unit (17.8A) PEG spacer arm.
Crosslink amines to sulfhydryls with this long crosslinker composed of NHS-ester and maleimide groups at ends of a stabilizing cyclohexane-containing, 15-atom spacer arm.
Crosslink amines to sulfhydryls with this crosslinker containing NHS-ester and pyridyldithiol groups at the ends of an aromatic, 6-atom (11.2A) spacer arm.
Crosslink amines to amines using these heavy (deuterated) and light matched pairs of a 7.7A, NHS-ester crosslinker for mass spectrometry analysis of protein interactions.
Crosslink amines to amines with dimethyl pimelimidate (CAS 58537-94-3); composed of imidoester groups and a membrane-permeable, 7-atom spacer arm.
Description | DMP |
---|---|
Méthode d’étiquetage | Chemical Labeling |
Perméabilité cellulaire | Yes |
Cleavable | No |
Type de produit | Crosslinker |
Longueur du bras espaceur | 9.2 Å |
Réactivité chimique | Amine-Amine |
Conditions d’expédition | Ambient |
Forme | Powder |
Contenu et stockage | Upon receipt store desiccated at 4°C. |
Pégylé | No |
Entretoise | Short (<10 Å) |
Poids moléculaire | 259.17 |
Format | Standard |
Type d’agent de réticulation | Homobifunctional |
Solubilité | Water |
Gamme de produits | Pierce™ |
Hydrosoluble | Yes |
Groupement de réactifs | Imidoester |
Crosslink amines to sulfhydryls with these crosslinkers containing NHS-ester and maleimide groups at ends of water-soluble polyethylene glycol spacer arms (17.6 to 95.2A).
Méthode d’étiquetage | Chemical Labeling |
---|---|
Perméabilité cellulaire | No |
Cleavable | No |
Type de produit | Crosslinker |
Longueur du bras espaceur | 24.6 Å |
Réactivité chimique | Amine-Sulfhydryl |
Conditions d’expédition | Ambient |
Forme | Solid |
Contenu et stockage | Upon receipt store desiccated at -20°C. |
Pégylé | Yes |
Entretoise | Medium (10 to 30 Å) |
Poids moléculaire | 513.5 |
Format | Standard |
Type d’agent de réticulation | Heterobifunctional |
Solubilité | Water |
Gamme de produits | Pierce™ |
Hydrosoluble | Yes |
Groupement de réactifs | Maleimide, NHS Ester |
Crosslink sulfhydryls to periodate-treated glycoprotein sugars with this long (17.9A) crosslinker containing maleimide and hydrazide groups.
Crosslink amines to sulfhydryls with this short, water-soluble crosslinker composed of sulfo-NHS-ester and maleimide groups at ends of an aromatic, 6-atom (7.3A) spacer arm.