DNA Vectors
Invitrogen™ pENTR™ 5'-TOPO™ TA Cloning™ Kit, with One Shot™ TOP10 Chemically Competent E. coli
Facilitates five-minute, one-step cloning of a Taq polymerase-amplified PCR product encoding a eukaryotic promoter of interest into a MultiSite Gateway entry vector with ≥85% efficiency
Invitrogen™ X-33, Pichia pastoris Yeast Strain
Optimize expression and recovery of protein of interest
Invitrogen™ Gateway™ pDEST™17 Vector
Destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins
Invitrogen™ Gateway™ pET-DEST42 Vector
Designed for rapid cloning with Gateway entry clone using lambda phage site-specific recombination and subsequent high-level prokaryotic expression controlled by strong bacteriophage T7 promoter
Invitrogen™ pcDNA™3.2-DEST Mammalian Expression Vector
Designed for high-level, constitutive expression in variety of mammalian cell lines
Invitrogen™ Gateway™ pDONR™221 Vector
Enables rapid cloning of one or more genes into virtually any protein expression system
Invitrogen™ MultiSite Gateway™ Pro Plus, for flexible cloning of up to four DNA fragments into a Gateway™ destination vector
For flexible cloning of up to four DNA fragments into Gateway destination vector
Invitrogen™ pIB/V5-His Vector Kit
The pIB/V5-His vector has several features that facilitate expression, analysis, and detection of recombinant protein in insect cells
Invitrogen™ pYES2/CT Yeast Expression Vector
S. cerevisiae expression vector derived from the parental pYES2 vector
Invitrogen™ Champion™ pET151 Directional TOPO™ Expression Kit with BL21 Star™ (DE3) One Shot™ Chemically Competent E. coli
Protein of interest can reach levels greater than 50 percent of total cellular protein during expression
Invitrogen™ Spheroplast Kit for Yeast
Contains qualified reagents for preparation of yeast spheroplasts
Invitrogen™ Multi-Copy Pichia Expression Kit
Contains pPIC3.5K, pPIC9K, and pAO815 vectors for production and selection of Pichia strains that contain more than one copy of gene of interest
Invitrogen™ Flp-In™ Core System
Contains necessary vectors that allow integration and expression of gene of interest in mammalian cells at specific genomic location
MilliporeSigma™ BrdU Cell Proliferation Assay Kits
BrdU Cell Proliferation Assay is a non-isotopic immunoassay for quantification of BrdU incorporation into newly synthesized DNA of actively proliferating cells. It is sensitive, rapid, and easy to perform.