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Invitrogen™ Bac-to-Bac™ Vector Kit

Catalog No. 10360014
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Catalog No. 10360014 Supplier Invitrogen™ Supplier No. 10360014
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Description

Includes

10μg of pFastBac1 vector and pFastBac 1-Gus control vector.

Designed for fast, small-scale production of recombinant baculovirus

  • Contains pFastBac™1 vector, as well expression control vector, intended for use as part of Bac-to-Bac Baculovirus Expression System, which enables efficent production of recombinant baculovirus for expression testing in insect cells
  • Relies on generation of recombinant baculovirus by site-specific transposition in E. colirather than homologous recombination in insect cells
  • pFastBac 1 vector provided by this kit offers strong polyhedrin promoter for protein expression and large multiple cloning site for simplified cloning
  • Time-saving expression bacmid: expression cassette of pFastBac vector recombines with parent bacmid in DH10BacE. coli
  • Competent Cells to form expression bacmid: bacmid is then transfected into insect cells for production of recombinant baculovirus particles
  • Easy colony screening
  • Parent bacmid in DH10Bac E. colicontains segment of lacZα gene
  • lacZα gene is disrupted upon transposition of expression cassette into bacmid allowing for blue/white selection of recombinants

Baculovirus Production and Titering, Insect Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

Promoter Polyhedrin
Product Type Vector Kit
Content And Storage Contains 10 μg of pFastBac™1 vector and pFastBac™ 1-Gus control vector.

Store at -20°C.
Protein Tag Untagged
Cloning Method Restriction Enzyme/MCS
Quantity 1 kit
Vector pFastBac
Product Line Bac-to-Bac

For Research Use Only. Not for use in diagnostic procedures.