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Invitrogen™ pLenti6/UbC/V5-DEST™ Gateway™ Vector
For lentiviral-based expression of a target gene in dividing and nondividing mammalian cells
Supplier: Invitrogen™ V49910
Includes: pLenti6/UbC/V5-DEST 6μg, pLenti6/UbC/V5-GW/lacZ Control vector: 10μg; Stbl3 Chemically Competent E. coli(Box 2), Stbl3 Cells: 21 x 50μL, pUC19 control DNA (10pg/μL): 50μL, S.O.C. Medium: 6mL.
Description
- The vector has the UbC promoter for driving constitutive but physiological levels of expression of the target gene and the blasticidin selection marker for stable selection
- Suited to long-term experiments
- Accurate titer of functional virus
- V5 epitope tag at C terminus
Cloning, Constitutive Expression, Gateway Cloning, Mammalian Expression, Protein Expression, Proteins, Expression, Isolation and Analysis, Viral Gene Delivery
Specifications
| Constitutive | |
| UbC | |
| Blasticidin | |
| V5 Epitope Tag | |
| 6 μg | |
| Gateway, ViraPower |
| Lentiviral | |
| Lentiviral Expression Vector | |
| Vector Kit (Box 1) pLenti6⁄UbC⁄V5-DEST™:6 μg, store at -20°C pLenti6⁄UbC⁄V5-GW⁄lacZ Control vector: 10 ug, store at -20°C One Shot™ Stbl3™ Chemically Competent E. coli(Box 2) Stbl3™ Cells: 21 x 50 μL, store at -80°C pUC19 control DNA (10 pg⁄μL): 50 μL, store at -80°C S.O.C. Medium: 6 mL, store at -80°C |
|
| Gateway | |
| pDEST, pLenti | |
| Viral Expression |
For Research Use Only. Not for use in diagnostic procedures.