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Invitrogen™ pBudCE4.1 Mammalian Expression Vector

Designed for the independent expression of two genes from a single plasmid in mammalian cells

Supplier: Invitrogen™ V53220

Catalog No. V53220

Quantity

Includes: 20μg each of pBudCE4.1 and pBudCE4.1/lacZ/CAT positive control vectors are supplied supercoiled and lyophilized.

Description

Using pBudCE4.1 to generate stable mammalian expression cell lines ensures that there is an equivalent copy number of each gene in cell; this can eliminate variable expression due to differences in gene copy number
Provides expression cassettes with the CMV promoter for high-level transcription of genes with optional c-myc epitope tag for rapid detection and 6xHis sequence for simple purification
Human EF-1α promoter for high-level expression of genes with optional V5 epitope tag for rapid detection and 6xHis sequence for simple purification
Sh ble (ZeoR) gene for efficient selection in both mammalian cells and E. coli with selection agent Zeocin™

Constitutive Expression, Mammalian Expression, Multi-Gene Expression, Protein Expression, Proteins, Expression, Isolation and Analysis

Specifications

Constitutive or Inducible System	Constitutive
Promoter	EF-1α, CMV
Selection Agent (Eukaryotic)	Zeocin™
Protein Tag	His Tag (6x), V5 Epitope Tag, c-Myc Epitope Tag
Quantity	20 μg
For Use With (Application)	Multi-Gene Expression

Delivery Type	Transfection
Product Type	Mammalian Expression Vector
Content And Storage	20 μg each of pBudCE4.1 and pBudCE4.1/lacZ/CAT positive control vectors are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.
Cloning Method	Restriction Enzyme/MCS
Vector	pBud

Product Suggestions

For Research Use Only. Not for use in diagnostic procedures.

Description