Invitrogen™ pTrcHis A, B, & C Bacterial Expression Vectors

Numéro de catalogue. V36020

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20 μg

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Numéro de catalogue.	Quantity
V36020	20 μg

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Numéro de catalogue. V36020 Fournisseur Invitrogen™ Code fournisseur V36020

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Description

All vectors are provided in suspension. A TOP10 E. coli stab and positive expression control is also provided.

Offer enhanced translation initiation and high-level expression in E. coli

These vectors feature high-level regulated transcription from the trc promoter
Enhanced translation efficiency of eukaryotic genes in E. coli
The lacO operator and lacIq repressor gene for transcriptional regulation in any E. coli strain
This particular vector offers N-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an anti-HisG antibody
N-terminal Xpress epitope for easy detection with an anti-Xpress antibody
Enterokinase cleavage site for removal of fusion tag

Bacterial Expression, Cloning, Gateway Cloning, Protein Expression, Proteins, Expression, Isolation and Analysis

Constitutive or Inducible System	Inducible
Inducing Agent	IPTG
Promoter	Trc, lacO
Product Type	Bacterial Expression Vector
Selection Agent (Eukaryotic)	None
Content And Storage	All vectors are provided in suspension. A TOP10 E. coli stab and positive expression control is also provided. Store vectors at -20°C. Store stab at room temperature. The vector is guaranteed stable for 6 months when properly stored.
Antibiotic Resistance Bacterial	Ampicillin (AmpR)
Cleavage	EK (Enterokinase) Recognition Site
Protein Tag	Thioredoxin
Cloning Method	Restriction Enzyme/MCS
Quantity	20 μg
Vector	pTrc
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For Research Use Only. Not for use in diagnostic procedures.