Modifying Enzymes
Corning™ Dispase
Bacillus-derived neutral metalloprotease that is recommended for recovering cells cultured on BD Matrigel™ Basement Membrane Matrix
Invitrogen™ TrueCut™ HiFi Cas9 Protein (1 μg/μL)
A high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut™ HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.
Invitrogen™ Bovine Serum Albumin
Suitable for use as enzyme stabilizer
MilliporeSigma™ Calbiochem™ Horseradish Peroxidase
Suitable for conjugation to proteins and antibodies and as cytochemical marker in immunohistochemical staining
Thermo Scientific™ T4 RNA Ligase (10 U/μL)
Catalyze the ATP-dependent formation of phosphodiester bonds between 5'-P and 3'-OH termini of oligonucleotides, single-stranded RNA and DNA.
Thermo Scientific™ T4 DNA Ligase (5 U/μL)
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA.
Invitrogen™ CIAP (Calf Intestinal Alkaline Phosphatase), 1 U/μL
Phosphomonoesterase that removes 3' and 5' phosphates from DNA and RNA
Thermo Scientific™ Exonuclease I (20 U/μL)
Degrade single-stranded DNA in a 3'→5' direction with Exonuclease I (ExoI), leaving 5'-terminal dinucleotides intact.
Thermo Scientific™ DNase I, RNase-free, HC (50 U/μL)
Endonuclease that nonspecifically cleaves single- and double-stranded DNA to release di-, tri-, and oligonucleotides with 5'-phosphate and 3'-OH groups.
MilliporeSigma™ Calbiochem™ Lysozyme, Human Neutrophil
Takes part in antibacterial activity of leukocytes
Gibco™ Dog MRP2 Vesicles
Dog MRP2 Vesicles are insect derived purified plasma membranes with inserted MRP2 transport protein (multidrug resistance-associated protein, ABCC2).
Thermo Scientific™ T4 beta-glucosyltransferase
Highly specific and fast T4 β-glucosyltransferase for 5-hmC modification analysis
MilliporeSigma™ Calbiochem™ Y-27632
Y-27632A, CAS 146986-50-7, is a cell-permeable, reversible, inhibitor of Rho kinases (Ki = 140 nM for p160ROCK). Enhances survival & cloning efficiency of ESC without affecting their pluripotency.