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Thermo Scientific™ RNase T1 (1000 U/μL)

Catalog No. FEREN0541
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Catalog No. FEREN0541 Supplier Thermo Scientific™ Supplier No. EN0541
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Description

RNase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues.

Thermo Scientific Rnase T1 is an endoribonuclease that specifically degrades single-stranded RNA at G residues (see Figure 1 in Supporting Data). It cleaves the phosphodiester bond between the 3'-guanylic residue and the 5'-OH residue of adjacent nucleotides with the formation of corresponding intermediate 2', 3'-cyclic phosphate. The reaction products are 3'-GMP and oligonucleotides with a terminal 3'-GMP.

Rnase T1 does not require metal ions for activity.

Applications

  • Removal of RNA from DNA preparations
  • RNA sequencing
  • Ribonuclease protection assays. Used in conjunction with RNase A
  • Removal of RNA from recombinant protein preparations
  • Determination of the level of RNA transcripts synthesized in vitro from DNA templates containing a "G-less cassette"

Specifications

Concentration 1000 U/μL
Enzyme RNase
Quantity 100,000 units
Product Type RNase T1

Frequently Asked Questions (FAQs)

How much Thermo Scientific RNase T1 should I use for complete digestion of RNA?

We would recommend 0.2 µL RNase T1 per µg of RNA in 50 mM Tris-HCl (pH 7.5) and 1 mM EDTA. Incubate at 37 degrees C for 30 min.

For Research Use Only. Not for use in diagnostic procedures.